Protective effect of Arbutus unedo aqueous extract in carrageenan-induced lung inflammation in mice
Protective effect of Arbutus unedo aqueous extract in carrageenan-induced lung inflammation in mice
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DOI：
10.1016/j.phrs.2007.12.005
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发表时间：
2008-02-01
影响因子：
9.3
通讯作者：
Cuzzocrea, Salvatore
中科院分区：
文献类型：
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作者：
Mariotto, Sofia;Esposito, Emanuela;Cuzzocrea, Salvatore
在本研究中，我们表明，杨梅的水提取物。(AuE)，一种地中海特有植物，广泛用作收敛剂、利尿剂和尿防腐剂，在体外下调一些炎症基因的表达，如编码诱导型一氧化氮合酶（NOS）和细胞内粘附分子（ICAM）-1的基因，对IFN-γ诱导的STAT 1活化和IL-6-β诱导的STAT 1活化都发挥抑制作用，为了进一步评估AuE在急性炎症动物模型中的作用，我们检查了AuE施用是否减弱由胸膜内注射角叉菜胶诱导的鼠的炎症反应。为此，我们研究了：（1）STAT 1/3激活，（2）胸膜渗出物中TNF-α、IL-1 β和IL-6的产生，（3）肺iNOS、考克斯-2和ICAM-1表达，（4）中性粒细胞浸润，（5）过氧亚硝酸盐对细胞蛋白质的硝化，（6）脂质过氧化，（7）前列腺素E2和亚硝酸盐/硝酸盐水平和（8）肺损伤。我们发现AuE强烈降低-调节由角叉菜胶在肺中诱导的STAT 3活化，伴随着与炎症相关的所有检查参数的衰减，提示STAT 3可能成为抗炎治疗的新分子靶点。这项研究表明，急性肺部炎症是显着减弱的治疗与金霉素。(C)2008爱思唯尔有限公司保留所有权利。
In the present study, we show that an aqueous extract of Arbutus unedo L. (AuE), a Mediterranean endemic plant widely employed as an astringent, diuretic and urinary anti-septic, in vitro down-regulates the expression of some inflammatory genes, such as those encoding inducible nitric oxide synthase (NOS) and intracellular adhesion molecule-(ICAM)-1, exerting a inhibitory action on both IFN-gamma-elicited STAT1 activation and IL-6-elicited STAT3 activation.To evaluate further the effect of AuE in animal models of acute inflammation, we examined whether AuE administration attenuates inflammatory response of murine induced by intrapleural injection of carrageenan. For this purpose we studied: (1) STAT1/3 activation, (2) TNF-alpha, IL-1 beta and IL-6 production in pleural exudate, (3) lung iNOS, COX-2 and ICAM-1 expression, (4) neutrophil infiltration, (5) the nitration of cellular proteins by peroxynitrite, (6) lipid peroxidation, (7) prostaglandin E2 and nitrite/nitrate levels and (8) lung injury.We show that AuE strongly down-regulates STAT3 activation induced in the lung by carrageenan with concomitant attenuation of all parameters examined associated with inflammation, suggesting that STAT3 should be a new molecular target for anti-inflammatory treatment. This study demonstrates that acute lung inflammation is significantly attenuated by the treatment with AuE. (C) 2008 Elsevier Ltd. All rights reserved.