Serological survey of Toxoplasma gondii in wild waterfowl in Chukotka, Kamchatka, Russia and Hokkaido, Japan
Serological survey of Toxoplasma gondii in wild waterfowl in Chukotka, Kamchatka, Russia and Hokkaido, Japan
复制标题
DOI：
10.1645/ge-1434.1
复制
发表时间：
2008-08-01
影响因子：
1.3
通讯作者：
Onuma, Misao
中科院分区：
文献类型：
--
作者：
Murao, Takehiro;Omata, Yoshitaka;Onuma, Misao
用ELISA法检测了22只家鸭的弓形虫抗体。此外，2004年和2005年在日本北海道带广对5种221只野鸭进行了血清学检测：Poecilorhyncha（n = 27）; acute（n = 58）; A.penelope（n = 16）; Crecca（n = 9）。还使用来自2个物种的197只野鹅的血清进行测定，白额雁（Anser albifrons，n = 162）和白额雁（Ans.蚕豆（n = 35）。在2003年和2005年之间，从3个不同的地区收集了鸟类：日本北海道宫岛沼湖，楚科奇自治州阿纳迪尔市周围地区和俄罗斯堪察加州Makobetukoa湖。基于未感染鸭的结果，ELISA截止值（OD）>= 0.395;确认为阳性的最终稀释比由最终滴度表示。实验诱导鸭的终滴度范围为1：400至1：3，200。抗T.日本野鸭221只，其中49只检出弓形虫：A.扁吻龙（22/74）; poecilorhyncha（2/15）; Poecilorhyncha（5/12）。197份野鹅样品中有32份血清学阳性，Ans. 2004年和2005年，日本宫岛沼地区的白额鲀分别为7/51和11/72，楚科奇地区的白额鲀为9/39。俄罗斯以及在安。fabalis（5/35），终滴度范围为1：100 ~ 1：3200。在免疫印迹中，A.platyrhynchus样品显示特异性IgG抗体与T.弓形虫泳道，即，在30和43 kDa处，但不在犬新孢子虫泳道中。在未检测到抗体活性的样品中未观察到特异性条带。这些结果表明，栖息在北海道、楚科奇和堪察加的野生水禽可能暴露于T。刚地
Antibodies to Toxoplasma gondii were assayed by ELISA in 22 experimentally inoculated domestic ducks. In addition a serological assay was carried out at Obihiro, Hokkaido, Japan, in 2004 and 2005, on 221 wild ducks of 5 species: Anas platyrhynchus (n = 111); A. poecilorhyncha (n = 27); A. acute (n = 58); A.penelope (n = 16); and A. crecca (n = 9). Assays were also conducted using sera from 197 wild geese of 2 species, i.e., Anser albifrons (n = 162) and Ans. fabalis (n = 35). Birds were collected between 2003 and 2005 from 3 different areas: Lake Miyajima-numa, Hokkaido, Japan, regions around Anadyr city of Chukotka antonomous okrug, and Lake Makobetukoa, Kamchatka oblast, Russia. The ELISA cutoff value (OD) was >= 0.395 based on results from uninfected ducks; the final dilution ratio recognized as positive was represented by the end titer. The end titer in the experimentally induced ducks ranged from 1:400 to 1:3,200. Antibodies to T. gondii were found in 49 of the 221 wild duck samples from Japan: A. platyrhynchus (22/74); A. poecilorhyncha (2/15); and A. poecilorhyncha (5/12). Thirty-two of 197 wild goose samples were seropositive, i.e., Ans. albifrons (7/51) in 2004 and (11/72) in 2005 in Miyajima-numa, Japan and 9/39 in Chukotka. Russia as well as in Ans. fabalis (5/35) in Kamchatka, for which the end titer ranged from 1:100 to 1:3,200. In immunoblotting, the A.platyrhynchus samples showed specific IgG antibody binding to several antigens in the T. gondii lane, i.e., at 30 and 43 kDa, but not in the Neospora caninum lane. No specific bands were noted in samples for which antibody activity was not detected. These results suggest that wild waterfowl inhabiting Hokkaido, Chukotka, and Kamchatka may be exposed to T. gondii.