The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana.
The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana.
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DOI：
10.1111/nph.12057
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发表时间：
2013-02
期刊：
影响因子：
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通讯作者：
Jian Li Yang;Wei Wei Chen-Wei;L. Chen;C. Qin;C. Jin;Yuan Shi;S. Zheng
中科院分区：
文献类型：
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作者：
Jian Li Yang;Wei Wei Chen-Wei;L. Chen;C. Qin;C. Jin;Yuan Shi;S. Zheng
在这里，我们报告的功能，一般调节因子，一般调节因子11（GRF 11），在铁（Fe）缺乏反应。研究了拟南芥grf 11突变体对铁胁迫的生理和分子响应。还分析了FER样铁缺乏诱导的转录后调节因子（FIT）的基因。此外，信号分子一氧化氮（NO）和铁缺乏反应之间的分子联系进行了进一步剖析。我们的研究结果表明，GRF 11是必要的诱导缺铁耐受机制。FIT蛋白可以与GRF 11的启动子结合，该启动子含有E-box基序。GRF 11也积极影响FIT转录，但对参与转录后调控的FIT基因没有影响。此外，NO正调控GRF 11的诱导后，发病的铁缺乏。我们建议，在铁缺乏的发病，诱导FIT的表达依赖于GRF 11，其作用下游的NO介导的铁缺乏反应。
Here we report the function of a general regulatory factor, GENERAL REGULATORY FACTOR11 (GRF11), in terms of the iron (Fe) deficiency response. Physiological and molecular responses of the loss-of-function Arabidopsis thaliana grf11 mutant to Fe supply were investigated. Genes involved in posttranscriptional regulation of FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) were also analyzed. In addition, the molecular link between the signaling molecule nitric oxide (NO) and Fe deficiency responses was further dissected. Our results suggest that GRF11 is necessary for induction of Fe-deficiency-tolerance mechanisms. The FIT protein can bind to the promoter of GRF11, which contains an E-box motif. GRF11 also positively affects FIT transcription but has no influence on the genes involved in posttranscriptional regulation of FIT. Furthermore, NO positively regulates GRF11 induction upon the onset of Fe deficiency. We propose that, upon the onset of Fe deficiency, induction of FIT expression is dependent on GRF11, which acts downstream of NO to mediate Fe deficiency responses.