UV-Tolerant Culturable Bacteria in an Asian Dust Plume Transported over the East China Sea
UV-Tolerant Culturable Bacteria in an Asian Dust Plume Transported over the East China Sea
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DOI：
10.4209/aaqr.2014.03.0067
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发表时间：
2015-04
影响因子：
4
通讯作者：
K. Hara;Daizhou Zhang;H. Matsusaki;Y. Sadanaga;Keisuke Ikeda;Sayuri Hanaoka;S. Hatakeyama
中科院分区：
文献类型：
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作者：
K. Hara;Daizhou Zhang;H. Matsusaki;Y. Sadanaga;Keisuke Ikeda;Sayuri Hanaoka;S. Hatakeyama
空气中的细菌沿着亚洲沙尘而分散，对大气特征、顺风生态系统和公共健康有着各种影响。本研究利用2010年12月东海上空亚洲沙尘过境和非沙尘条件下的飞机观测资料，采用基于培养的基因分析方法对沙尘中可培养细菌的组成进行了研究。16S rRNA基因分析表明，从运尘中分离的34株培养菌可分为芽孢杆菌目（Bacillales）和放线菌目（Actinomycetales）。高耐受性内孢子形成细菌（25株）占主导地位的组合物。另一方面，所有非内生孢子形成细菌（9个分离株）均为革兰氏阳性菌，具有高鸟嘌呤和胞嘧啶含量，具有显著的紫外线（UV）抗性。因此，分离的可培养细菌是对暴露于UV辐射具有一定耐受性的菌株。虽然大气是细菌生存的极端环境，但亚洲灰尘可以促进可培养细菌的大气扩散，这些细菌对恶劣的周围空气环境具有抵抗力。
Airborne bacteria are dispersed along with Asian dust and have various influences on atmospheric characteristics, downwind ecosystems, and public health. In this study, aircraft observations were conducted during Asian dust passage and non-dust conditions over the East China Sea in December 2010, and the composition of culturable bacteria within the dust was investigated using culture-based gene analysis. The 16S rRNA gene analysis revealed that the 34 cultured strains isolated from the transported dust could be grouped into Bacillales and Actinomycetales. Highly tolerant endospore-forming bacteria (25 isolates) were predominant in the composition. On the other hand, all non-endospore-forming bacteria (9 isolates) were gram-positive bacteria with high guanine and cytosine contents, which have substantial ultraviolet (UV) resistance. Thus, the isolated culturable bacteria were strains having some tolerance for exposure to UV radiation. Although the atmosphere is an extreme environment for bacterial survival, Asian dust can facilitate atmospheric dispersion of culturable bacteria that are resistant to the harsh ambient air environment.