Lipid Droplets Define a Sub-Population of Breast Cancer Stem Cells
Lipid Droplets Define a Sub-Population of Breast Cancer Stem Cells
复制标题
脂滴定义乳腺癌干细胞亚群
DOI：
10.3390/jcm9010087
复制
发表时间：
2020-01-01
影响因子：
3.9
通讯作者：
Havas, Kristina M.
中科院分区：
文献类型：
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作者：
Hershey, Benjamin J.;Vazzana, Roberta;Havas, Kristina M.
肿瘤复发现在是乳腺癌相关死亡的主要原因。这些复发被认为是由在初始治疗干预中存活的残留癌症干细胞引起的。因此，需要全面了解癌症干细胞生物学，以产生更有效的治疗方法。在这里，我们调查脂质代谢失调和乳腺癌干细胞之间的关联。特别关注脂滴，我们发现在一组乳腺细胞系中脂滴数量与干性相关。使用为这项研究开发的基于流式细胞术的方法，我们建立了一种从总人群中分离具有增强脂滴负荷的细胞的方法，并显示它们富含癌症干细胞。此外，脂肪酸代谢的药理学靶向揭示了细胞系亚群中的代谢成瘾。我们的研究结果强调了脂质代谢在维持乳腺癌干细胞库中的关键作用，因此，建议将其作为潜在的治疗靶点。
Tumor recurrence is now the leading cause of breast cancer-related death. These recurrences are believed to arise from residual cancer stem cells that survive initial therapeutic intervention. Therefore, a comprehensive understanding of cancer stem cell biology is needed to generate more effective therapies. Here we investigate the association between dysregulation of lipid metabolism and breast cancer stem cells. Focusing specifically on lipid droplets, we found that the lipid droplet number correlates with stemness in a panel of breast cell lines. Using a flow cytometry-based method developed for this study, we establish a means to isolate cells with augmented lipid droplet loads from total populations and show that they are enriched in cancer stem cells. Furthermore, pharmacological targeting of fatty acid metabolism reveals a metabolic addiction in a subset of cell lines. Our results highlight a key role for the lipid metabolism in the maintenance of the breast cancer stem cell pool, and as such, suggest it as a potential therapeutic target.