Lung surfactant proteins in the early human placenta
Lung surfactant proteins in the early human placenta
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早期人类胎盘中的肺表面活性蛋白
DOI：
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发表时间：
2009
影响因子：
2.3
通讯作者：
R. Demir
中科院分区：
文献类型：
--
作者：
L. Sati;Y. Seval;R. Demir
肺表面活性物质是磷脂和四种表面活性物质相关蛋白（SP-A、SP-B、SP-C和SP-D）的复杂混合物。SP-A和SP-D的生物学功能主要是双重的，即表面活性剂稳态和宿主防御。疏水表面活性剂蛋白SP-B和SP-C是通过促进表面活性剂磷脂沿着肺泡表面的快速吸附来实现表面活性剂的最佳表面张力降低特性所必需的。尽管这些发现很有希望，但对这些蛋白质的肺外分布知之甚少。因此，在本研究中，SP-A，SP-B，SP-C和SP-D在早期人胎盘中的存在进行了研究。从在正常妊娠期间接受刮宫术的女性获得妊娠早期胎盘组织（22-56天）。在平行的组织切片中，波形蛋白，细胞角蛋白-7和CD-68免疫染色分别用于鉴定间充质细胞，滋养层细胞和Hofbauer细胞。免疫组化结果显示，SP-A、SP-B、SP-C和SP-D在绒毛滋养层、滋养层细胞柱、基质细胞、Hofbauer细胞、血管生成细胞索和血管内皮细胞中均有不同程度的表达。胎儿造血细胞表现出一个可变的染色模式，所有四个表面活性剂蛋白，从没有到强强度。组织提取物的Western印迹证实了我们的IHC结果。在早期人类胎盘表面活性剂糖蛋白的存在下，可能会产生一个非常重要的功能，表面活性剂在妊娠早期，并使进一步研究的作用，表面活性剂在各种妊娠并发症。
Pulmonary surfactant is a complex mixture of phospholipids and four surfactant-associated proteins (SP-A, SP-B, SP-C and SP-D). The biological functions of SP-A and SP-D are primarily twofold, namely surfactant homeostasis and host defense. The hydrophobic surfactant proteins, SP-B and SP-C, are required for achieving the optimal surface tension reducing properties of surfactant by promoting the rapid adsorption of surfactant phospholipids along the alveolar surface. Despite the promising findings, only little is known about the extrapulmonary distribution of these proteins. Therefore, in this study, the presence of SP-A, SP-B, SP-C and SP-D in early human placenta has been investigated. First-trimester placental tissues (22–56 days) were obtained from women undergoing curettage during normal pregnancies. In parallel tissue sections, vimentin, cytokeratin-7 and CD-68 immunostainings were used for the identification of mesenchymal cells, trophoblast cells and Hofbauer cells, respectively. According to immunohistochemistry (IHC) results, SP-A, SP-B, SP-C and SP-D immunoreactivities with different staining intensities were observed in trophoblastic layers of chorionic villous tree, trophoblastic cell columns, stromal cells, Hofbauer cells, angiogenic cell cords and vascular endothelium. Fetal hematopoietic cells showed a variable staining pattern for all four surfactant proteins ranging from none to strong intensity. Western blotting of tissue extracts confirmed our IHC results. The presence of surfactant glycoproteins in early human placenta may yield a very important feature of surfactants during first trimester and enables further studies of the role of surfactants in various pregnancy complications.
含有碱性磷酸酶和其他消化酶的小肠表面活性剂样颗粒的分离和表征。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eliakim,R;DeSchryver-Kecskemeti,K;Nogee,L;Stenson,WF;Alpers,DH
通讯作者：
Alpers,DH
皮质醇诱导表面活性蛋白 A 表达促进人绒毛膜滋养层中前列腺素的合成。
DOI：
10.1210/jc.2006-1472
发表时间：
2006
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Sun,Kang;Brockman,Diane;Campos,Begona;Pitzer,Brad;Myatt,Leslie
通讯作者：
Myatt,Leslie
DOI：
10.1152/ajplung.00011.2003
发表时间：
2003-09-01
影响因子：
4.9
作者：
Melton, KR;Nesslein, LL;Weaver, TE
通讯作者：
Weaver, TE