Monoclonal antibodies to DNA modified by 8-methoxypsoralen and ultraviolet A light.
Monoclonal antibodies to DNA modified by 8-methoxypsoralen and ultraviolet A light.
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经过 8-甲氧基补骨脂素和紫外线 A 光修饰的 DNA 单克隆抗体。
DOI：
10.1093/nar/13.7.2533
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发表时间：
1985
影响因子：
14.9
通讯作者：
Edelson,RL
中科院分区：
文献类型：
--
作者：
Santella,RM;Dharmaraja,N;Gasparro,FP;Edelson,RL
已经开发了一组单克隆抗体，其特异性地识别由8-甲氧基卟啉（8-MOP）和紫外线A光（320-400 nm）（UVA）修饰的DNA。这些抗体已通过酶联免疫吸附试验（ELISA）的灵敏度和特异性进行了表征。在用最敏感的抗体进行的竞争性ELISA中，在17 fmole 8-MOP-DNA光加合物时发生50%的抗体结合抑制。每10个碱基可以可靠地检测到一个加合物。与4′-氨甲基-4，5，8-三甲基胡椒烯和4′，5-二甲基当归素修饰的DNA以及经8-MOP和UVA处理的细胞DNA也有一定的交叉反应。通过使用8-MOP poly（dA-dT）.poly（dA-dT）酶消化物的HPLC级分进行竞争性抑制研究，证明其中一种抗体的主要特异性为1′，5′胸腺嘧啶单加合物。这些抗体应允许在各种体外系统以及临床暴露于8-MOP和UVA的人中定量加合物水平。
A panel of monoclonal antibodies have been developed which specifically recognize DNA modified by 8-methoxypsoralen (8-MOP) and ultraviolet A light (320–400 nm) (UVA). These antibodies have been characterized as to sensitivity and specificity by an enzyme linked immunosorbent assay (ELISA). In a competitive ELISA with the most sensitive antibody, 50% inhibition of antibody binding occurred at 17 fmole 8-MOP-DNA photo adducts. One adduct per 10 bases could be reliably detected. There was also some antibody cross-reactivity with DNAs modified by 4′ aminomethyl-4, 5, 8-trimethylpsoralen and 4′, 5-dimethylangelicin as well as DNA isolated from cells treated with 8-MOP and UVA. The primary specificity of one of the antibodies was shown to be the 1′, 5′ thymine monoadduct by competitive inhibition studies using HPLC fractions of an enzymatic digest of 8-MOP poly(dA-dT).poly(dA-dT). These antibodies should allow the quantitation of adduct levels in various in vitro systems as well as humans exposed clinically to 8-MOP and UVA.