Effect of Triptolide on T-Cell Receptor Beta Variable Gene mRNA Expression in Rats With Collagen-Induced Arthritis
Effect of Triptolide on T-Cell Receptor Beta Variable Gene mRNA Expression in Rats With Collagen-Induced Arthritis
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DOI：
10.1002/ar.22479
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发表时间：
2012-06-01
影响因子：
2
通讯作者：
Xu, Yudong
中科院分区：
文献类型：
--
作者：
Wang, Jixi;Wang, Aibing;Xu, Yudong
雷公藤内酯醇（TP）已被用于治疗类风湿性关节炎（RA），但其作用机制尚不清楚。T细胞活化和相关细胞因子的释放似乎是RA发病机制中的主要因素。T细胞受体（TCR）可变基因（V基因）片段的过表达可引起自身反应性T细胞的活化和浸润。本研究探讨TP对胶原诱导性关节炎（CIA）大鼠的影响。ELISA法检测血清白细胞介素10（IL-10）水平。与CIA组相比，TP治疗组的IL-10水平更高。实时定量聚合酶链反应证实，CIA组中TCR V β（BV）15和TCR BV 19的表达增加，而TP治疗组中，表达降低。在这项研究中，TP被发现提高IL-10水平，降低TCR BV 15和TCR BV 19的表达水平。这些变化可能有助于解释TP治疗RA的有效性。Anat Rec，2012. (c)2012 Wiley Periodicals，Inc.
Triptolide (TP) has been used in the treatment of rheumatoid arthritis (RA), but its mechanism of action is not understood. T-cell activation and associated release of cytokines appear to be major factors in the pathogenesis of RA. The overexpression of T-cell receptor (TCR) variable gene (V gene) fragments can cause the activation and infiltration of autoreactive T cells. This study examines the effects of TP on rats with collagen-induced arthritis (CIA). The levels of interleukin-10 (IL-10) in the serum were examined with ELISA. Compared to the CIA group, the levels of IL-10 were greater in the TP treatment group. Real-time quantitative polymerase chain reaction confirmed that the expression of TCR V beta (BV) 15 and TCR BV19 was increased in the CIA group, whereas in the TP treatment group, the expression was decreased. In this study, TP was found to enhance IL-10 levels and decrease the expression levels of TCR BV15 and TCR BV19. These changes might help explain the effectiveness of TP in the treatment of RA. Anat Rec, 2012. (c) 2012 Wiley Periodicals, Inc.