Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy
Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy
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DOI：
10.1039/d0sm00943a
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发表时间：
2020-10-21
期刊：
影响因子：
3.4
通讯作者：
Dimova, Rumiana
中科院分区：
文献类型：
--
作者：
Faizi, Hammad A.;Reeves, Cody J.;Dimova, Rumiana
一种广泛使用的方法来测量双层膜的抗弯刚度是波动光谱，它分析了热驱动的膜波动的巨大单层囊泡记录的相位差或共聚焦显微镜。在这里，我们使用这两种技术分析相同的囊泡的波动，并获得一致的弯曲模量值。我们讨论了可能导致差异的因素。
A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.