Neuroprotective Effects of Constituents of the Root Bark of Dictamnus dasycarpus in Mouse Hippocampal Cells
Neuroprotective Effects of Constituents of the Root Bark of Dictamnus dasycarpus in Mouse Hippocampal Cells
复制标题
白鲜肉根皮成分对小鼠海马细胞的神经保护作用
DOI：
10.1007/s12272-010-0818-9
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发表时间：
2010-08-01
影响因子：
6.7
通讯作者：
Kim, Youn-Chul
中科院分区：
文献类型：
--
作者：
Jeong, Gil-Saeng;Byun, Erisa;Kim, Youn-Chul
Glutamate-induced oxidative injury causes neuronal degeneration related to many central nervous system diseases, such as Parkinson's disease, Alzheimer's disease, epilepsy and ischemia. The bioassay-guided fractionation of the EtOH extract of the root bark of Dictamnus dasycarpus Trucz. provided one neuroprotective limonoid, obacunone, together with a degraded limonoid, fraxinellone and two alkaloids, dictamine and haplopine. At concentrations of 100-150 mu M, obacunone showed the potent neuroprotective effects on glutamate-induced neurotoxicity and induced the expression of heme oxygenase (HO)-1 in the mouse hippocampal HT22 cells. In addition, we found that obacunone increased p38 MAPK phosphorylation and induced HO-1 expression via p38 MAPK pathway. These results suggest that obacunone isolated from the root bark of D. dasycarpus increases cellular resistance to glutamate-induced oxidative injury in mouse hippocampal HT22 cells, presumably through the p38 MAPK pathway-dependent HO-1 expression. These results suggest that obacunone could be the effective candidates for the treatment of ROS-related neurological diseases.
