Using a nanoelectrospray-differential mobility spectrometer-mass spectrometer system for the analysis of oligosaccharides with solvent selected control over ESI aggregate ion formation
Using a nanoelectrospray-differential mobility spectrometer-mass spectrometer system for the analysis of oligosaccharides with solvent selected control over ESI aggregate ion formation
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DOI：
10.1016/j.jasms.2006.10.008
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发表时间：
2007-03-01
影响因子：
3.2
通讯作者：
Nazarov, Erkinjon G.
中科院分区：
文献类型：
--
作者：
Levin, Daren S.;Vouros, Paul;Nazarov, Erkinjon G.
差分迁移谱（DMS），通常也称为高场非对称波形离子迁移谱（FAIMS），是用于气相离子分离的快速发展的技术。DMS与质谱（MS）的接口提供了优于单独使用质谱的潜在优势。这样的优点包括改进质谱信号/噪声，正交/互补离子分离质谱，增强离子和络合结构分析，和快速分析物定量的潜力。在这份报告中，我们证明了我们的nanoESI-DMS-MS系统的成功使用，与甲醇漂移气体改性剂，分离寡糖。描述了ESI形成寡糖聚集体离子的趋势及其对nanoESI-DMS-MS寡糖分析的负面影响。此外，我们还证明了样品溶剂选择对控制nanoESI寡糖聚集体离子形成及其对聚糖电离和DMS分离的影响的重要性。四氯乙烷/甲醇溶剂溶液的成功使用，以减少ESI寡糖聚集体离子的形成，同时有效地形成一个占主导地位的MH+分子离子。通过减少聚集体离子形成以有利于主要MH+离子，DMS选择性和特异性得到改善。除DMS外，我们预计聚集体离子复杂性的降低将有利于其他后ESI分离技术（如质谱和离子迁移率）的寡糖分析。通过使用四氯乙烷/甲醇溶剂对MH+分子离子形成进行溶剂选择控制，也有望增强聚糖的MS/MS结构表征分析。
Differential mobility spectrometry (DMS), also commonly referred to as high field asymmetric waveform ion mobility spectrometry (FAIMS) is a rapidly advancing technology for gas-phase ion separation. The interfacing of DMS with mass spectrometry (MS) offers potential advantages over the use of mass spectrometry alone. Such advantages include improvements to mass spectral signal/noise, orthogonal/complementary ion separation to mass spectrometry, enhanced ion and complexation structural analysis, and the potential for rapid analyte quantitation. In this report, we demonstrate the successful use of our nanoESI-DMS-MS system, with a methanol drift gas modifier, for the separation of oligosaccharides. The tendency for ESI to form oligosaccharide aggregate ions and the negative impact this has on nanoESI-DMS-MS oligosaccharide analysis is described. In addition, we demonstrate the importance of sample solvent selection for controlling nanoESI oligosaccharide aggregate ion formation and its effect on glycan ionization and DMS separation. The successful use of a tetrachloroethane/methanol solvent solution to reduce ESI oligosaccharide aggregate ion formation while efficiently forming a dominant MH+ molecular ion is presented. By reducing aggregate ion formation in favor of a dominant MH+ ion, DMS selectivity and specificity is improved. In addition to DMS, we would expect the reduction in aggregate ion complexity to be beneficial to the analysis of oligosaccharides for other post-ESI separation techniques such as mass spectrometry and ion mobility. The solvent selected control over MH+ molecular ion formation, offered by the use of the tetrachloroethane/methanol solvent, also holds promise for enhancing MS/MS structural characterization analysis of glycans.