Molecular areas of phospholipids as determined by 2H NMR spectroscopy. Comparison of phosphatidylethanolamines and phosphatidylcholines.
Molecular areas of phospholipids as determined by 2H NMR spectroscopy. Comparison of phosphatidylethanolamines and phosphatidylcholines.
复制标题
通过 2H NMR 光谱测定磷脂的分子面积。
DOI：
10.1016/s0006-3495(91)82203-2
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发表时间：
1991
影响因子：
3.4
通讯作者：
Brown,MF
中科院分区：
文献类型：
--
作者：
Thurmond,RL;Dodd,SW;Brown,MF
生物膜中脂质多样性的作用是生物化学中尚未解决的主要问题之一。一个可能重要的参数是每个脂质分子所占据的平均横截面积，这可能与非双层结构的形成和膜蛋白功能有关。我们使用 2H NMR 光谱来比较 1,2-二全氘代棕榈酰-sn-甘油-3-磷酸乙醇胺 (DPPE-d62) 和 1,2-二全氘代棕榈酰-sn-甘油-3-磷酸胆碱 (DPPC-d62) 在 L α 相中的性质。我们发现 DPPE 比 DPPC 具有更大的链段有序度，并且这种有序度的增加与 DPPE 中每个酰基链的较小面积有关。使用用于酰基链构型统计的简单金刚石晶格模型以及膨胀数据来计算平均横截链面积的值。每个分子平均面积的结果与低角度 X 射线衍射研究的结果相当。
The role of lipid diversity in biomembranes is one of the major unsolved problems in biochemistry. One parameter of possible importance is the mean cross-sectional area occupied per lipid molecule, which may be related to formation of nonbilayer structures and membrane protein function. We have used 2H NMR spectroscopy to compare the properties of 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-d62) and 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphocholine (DPPC-d62) in the L alpha phase. We find that DPPE has greater segmental order than DPPC, and that this increase in order is related to the smaller area per acyl chain found for DPPE. Values of the mean cross-sectional chain area are calculated using a simple diamond lattice model for the acyl chain configurational statistics, together with dilatometry data. The results obtained for the mean area per molecule are comparable with those from low angle x-ray diffraction studies.