Brazilian isolates of Trypanosoma (Megatrypanum) theileri:: diagnosis and differentiation of isolates from cattle and water buffalo based on biological characteristics and randomly amplified DNA sequences
Brazilian isolates of Trypanosoma (Megatrypanum) theileri:: diagnosis and differentiation of isolates from cattle and water buffalo based on biological characteristics and randomly amplified DNA sequences
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DOI：
10.1016/s0304-4017(03)00236-x
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发表时间：
2003-10-20
影响因子：
2.6
通讯作者：
Teixeira, MMG
中科院分区：
文献类型：
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作者：
Rodrigues, AC;Campaner, M;Teixeira, MMG
我们从巴西东南部圣保罗州的牛和水牛中检测并培养了泰氏锥虫（Megatrypanum）分离株，通过比较形态、生长和分子特征对其进行了表征。虽然从牛和布法罗分离的形态上难以区分，但它们的生长特征不同。随机扩增多态性DNA（RAPD）模式的基础上的树状图表明，密切的遗传关系之间的所有菌株从这两个物种，这是所有紧密聚在一起，远离Megatrypanum物种从野生哺乳动物。此外，T.泰勒氏菌群明显分为两个宿主相关组。结果显示，在所有T.来自牛和布法罗的泰勒氏锥虫，但在其他13种锥虫中未检测到，被用作常规T.泰勒氏特异性PCR测定。我们还定义了RAPD片段（Tthc 606和Tthb 606），区分牛从布法罗菌株。因此，不同的生长特征和遗传变异区分菌株从牛和水牛相同的地理来源，这表明这些菌株与其宿主物种的关联。本文报道的布法罗锥虫是我国首次发现的锥虫。本研究采用生物学和分子生物学方法，对亚洲布法罗（Bubalus bubalis）的类泰勒氏菌进行连续培养，并与黄牛分离株进行比较。(C)2003 Elsevier B.V.保留所有权利。
We detected and cultivated isolates of Trypanosoma (Megatrypanum) theileri from cattle and water buffaloes in Sao Paulo state, southeastern Brazil, which were characterized by comparing morphological, growth and molecular features. Although isolates from cattle and water buffalo were morphologically indistinguishable, they differed in their growth characteristics. A dendrogram based on randomly amplified polymorphic DNA (RAPD) patterns indicated close-genetic relationships among all isolates from both species, which were all tightly clustered together and distant from Megatrypanum species from wild mammals. In addition, isolates within the T. theileri-cluster were clearly segregated into two host-associated groups. The sequence of a synapomorphic RAPD-derived DNA fragment (Tth625), which was shared by all T. theileri trypanosomes from cattle and buffalo but not detected in any of 13 other trypanosome species, was used as target for a conventional T. theileri-specific PCR assay. We also defined RAPD fragments (Tthc606 and Tthb606) that distinguished cattle from buffalo isolates. Thus, distinct growth features and genetic variability distinguished between isolates from cattle and water buffaloes of the same geographic origin, suggesting an association of these isolates with their host species. The trypanosomes from water buffalo reported here are the first T. theileri-like isolates from the Asiatic buffalo (Bubalus bubalis) to be continuously cultured and compared with cattle isolates using biological and molecular methods. (C) 2003 Elsevier B.V. All rights reserved.