REGULATION OF HYDROGEN-PEROXIDE IN EYE HUMORS - EFFECT OF 3-AMINO-1H-1,2,4-TRIAZOLE ON CATALASE AND GLUTATHIONE PEROXIDASE OF RABBIT EYE
REGULATION OF HYDROGEN-PEROXIDE IN EYE HUMORS - EFFECT OF 3-AMINO-1H-1,2,4-TRIAZOLE ON CATALASE AND GLUTATHIONE PEROXIDASE OF RABBIT EYE
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DOI：
10.1016/0304-4165(77)90284-7
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
BHUYAN, DK
中科院分区：
文献类型：
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作者：
BHUYAN, KC;BHUYAN, DK
在虹膜、睫状体、视网膜、角膜上皮、角膜内皮、透镜囊-上皮和去囊透镜中测量过氧化氢酶（H2 O2：H2 O2氧化还原酶，EC 1.11.1.6）和GSH [谷胱甘肽]过氧化物酶（GSH：H2 O21.11.1.9）的活性。3-Amino-1H-1，2，4-triazole是一种特异性的过氧化氢酶抑制剂，也是一种有效的白内障发生剂。家兔静脉注射1 g 3-氨基三唑/kg体重后1-6 h，这些组织中的过氧化氢酶活性明显受到抑制。这与房水和玻璃体液中H2 O2浓度增加2-3倍有关。随着注射后时间的推移，眼组织的过氧化氢酶活性逐渐恢复正常，增加的过氧化物浓度恢复到生理水平。在该条件下，上述眼组织的GSH过氧化物酶活性没有改变，透镜的GSH和蛋白巯基没有改变，并且房水和玻璃体液的抗坏血酸没有显著改变。眼组织过氧化氢酶明显调节眼体液中内源性H_2O_2至生理水平。H_2O_2可能是3-氨基三唑诱发白内障的触发因素。
Activities of catalase (H2O2:H2O2 oxidoreductase, EC 1.11.1.6) and GSH [glutathione] peroxidase (GSH:H2O2 oxidoreductase, EC 1.11.1.9) were measured in iris, ciliary body, retina, corneal epithelium, corneal endothelium, lens capsule-epithelium and decapsulated lens. 3-Amino-1H-1,2,4-triazole is a specific inhibitor of catalase and a potent cataractogenic agent. There was marked inhibition of catalase activity in these tissues 1-6 h after the administration of a single i.v. dose of 1 g 3-aminotriazole/kg body weight in rabbit. This was associated with a 2-3-fold increase in the H2O2 concentrations of aqueous humor and vitreous humor. The increased peroxide concentrations were restored to the physiological levels as the catalase activity of eye tissues gradually returned to normal with time after injection. Under the conditions, GSH peroxidase activity of the aforementioned eye tissues was unaltered, GSH and protein sulfhydryl of lens were not changed, and ascorbic acid of aqueous humor and vitreous humor was not significantly altered. Catalase of eye tissues apparently regulates the endogenous H2O2 in eye humors to the physiological level. H2O2 may be the triggering factor in cataract induced by 3-aminotriazole.