Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin
Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin
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DOI：
10.1034/j.1600-0625.2002.110502.x
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发表时间：
2002-10-01
影响因子：
3.6
通讯作者：
Uitto, J
中科院分区：
文献类型：
--
作者：
El-Domyati, M;Attia, S;Uitto, J
皮肤老化是一种复杂的生物学现象，影响皮肤的不同成分。为了比较内在和外在衰老过程的影响，从健康志愿者的阳光暴露和保护皮肤中收集了总共83个活检样本，代表了从1岁到9岁（6-84岁）的几十年。常规组织病理学结合计算机辅助图像分析用于评估表皮变化。免疫过氧化物酶技术与抗I型和III型胶原和弹性蛋白的抗体被用来定量评估胶原和弹性纤维的变化，并通过透射电子显微镜检查其超微结构。表皮厚度被认为是恒定的，在不同的几十年在阳光下暴露和保护皮肤，但是，它是显着更大的阳光下暴露的皮肤（P = 0.0001）。在受保护的皮肤中，I型和III型胶原蛋白染色仅在第8个十年后改变，而在阳光暴露的皮肤中，相对染色强度分别从第1个十年的82.5%和80.4%显著降低至第9个十年的53.2%和44.1%（P = 0.0004和0.0008）。在面部皮肤中，胶原纤维结构在第40年后出现紊乱。弹性蛋白在保护皮肤中的染色强度从第一个十年的49.2%显著降低到第九个十年的30.4%（P = 0.05），而在阳光暴露皮肤中的强度从第一个十年的56.5%逐渐增加到第九个十年的75.2%（P = 0.001）。面部皮肤中积聚的弹性蛋白形态异常，似乎占据了失去的胶原蛋白区域。总的来说，老化过程，无论是内在的还是外在的，对皮肤中的胶原蛋白和弹性纤维都有定量和定性的影响。
Cutaneous aging is a complex biological phenomenon affecting the different constituents of the skin. To compare the effects of intrinsic and extrinsic aging processes, a total of 83 biopsies were collected from sun-exposed and protected skin of healthy volunteers representing decades from the 1st to the 9th (6-84 years of age). Routine histopathology coupled with computer-assisted image analysis was used to assess epidermal changes. Immunoperoxidase techniques with antibodies against type I and type III collagens and elastin were used to quantitatively evaluate changes in collagen and elastic fibers and their ultrastructure was examined by transmission electron microscopy. Epidermal thickness was found to be constant in different decades in both sun-exposed and protected skin; however, it was significantly greater in sun-exposed skin (P = 0.0001). In protected skin, type I and III collagen staining was altered only after the 8th decade, while in sun-exposed skin the relative staining intensity significantly decreased from 82.5% and 80.4% in the 1st decade to 53.2% and 44.1% in the 9th decade, respectively (P = 0.0004 and 0.0008). In facial skin the collagen fiber architecture appeared disorganized after the 4th decade. The staining intensity of elastin in protected skin significantly decreased from 49.2% in the 1st decade to 30.4% in the 9th decade (P = 0.05), whereas in sun-exposed skin the intensity gradually increased from 56.5% in the 1st decade to 75.2% in the 9th decade (P = 0.001). The accumulated elastin in facial skin was morphologically abnormal and appeared to occupy the areas of lost collagen. Collectively, the aging processes, whether intrinsic or extrinsic, have both quantitative and qualitative effects on collagen and elastic fibers in the skin.