SELECTED-ION ACCUMULATION OF NONCOVALENT COMPLEXES IN A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETER
SELECTED-ION ACCUMULATION OF NONCOVALENT COMPLEXES IN A FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETER
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DOI：
10.1002/jms.1190300119
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发表时间：
1995-01-01
影响因子：
2.3
通讯作者：
SMITH, RD
中科院分区：
文献类型：
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作者：
BRUCE, JE;VANORDEN, SL;SMITH, RD
电喷雾电离-傅里叶变换离子回旋共振 (ESI-FTICR) 质谱仪与选择离子累积 (SIA) 技术结合使用，研究其在溶液中非共价配合物气相行为研究中的实用性。 SIA 被证明可以选择性积累弱结合物质，从而显着增强可达到的信噪比和动态范围。四极激发的频率响应首先通过选择性积累大的、高度加合的、高电荷分子（例如牛白蛋白和牛白蛋白二聚体）的单电荷态来确定。在所采用的条件下，发现响应（选择性累积）窄至 500 Hz。此外，SIA被发现足够温和，可以积累特异性和非特异性弱非共价复合物，例如在碱性溶液中观察到的肌红蛋白-血红素复合物以及肌红蛋白和单一氨基酸（例如色氨酸）的复合物。这一结果可能是由于在高压累积事件期间连续应用四极激励，防止了磁控管运动的任何显着增长，并且随后允许相互转换仅形成小的回旋加速器半径。我们还演示了在离子积累期间同时应用 SIA 和偶极持续偏共振照射 (SORI)，以便选择性积累解离产物。 SIA/SORI 组合有望通过允许沿着给定解离途径的任何步骤选择性积累任何解离产物，从而极大地扩展 (MS)(n) 能力。
An electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometer has been used in conjunction with the technique of selected-ion accumulation (SIA) to investigate its utility for the study of the gas phase behavior of noncovalent complexes from solution. SIA is demonstrated to provide the selective accumulation of weakly bound species, providing a significant enhancement in the attainable signal-to-noise ratio and dynamic range, The frequency response of quadrupole excitation was first determined by selectively accumulating single charge states of large, highly adducted, highly charged molecules such as bovine albumin and bovine albumin dimer. Under the conditions employed, the response (selective accumulation) was found to be as narrow as 500 Hz. additionally the SIA was found to be sufficiently gentle to allow accumulation of both specific and nonspecific weak noncovalent complexes, such as the myoglobin-heme complex observed in basic solutions and complexes of myoglobin and single amino acids, such as tryptophan. This result is probably due to the continual application of quadrupole excitation during the high pressure accumulation event, preventing any significant growth of magnetron motion, and subsequently, allowing interconversion to form only small cyclotron radii. We also demonstrate the simultaneous application of SIA with dipolar sustained off-resonance irradiation (SORI) during the ion accumulation period so as to allow selective accumulation of dissociation products. The SIA/SORI combination promises to greatly extend (MS)(n) capabilities by allowing the selective accumulation of any dissociation product in any step along a given dissociation pathway.