Immunohistochemical studies of protein kinase D (PKD) 2 expression in malignant human lymphomas.
Immunohistochemical studies of protein kinase D (PKD) 2 expression in malignant human lymphomas.
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发表时间：
2006-09
影响因子：
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通讯作者：
L. Kovalevska;O. Yurchenko;L. Shlapatska;G. G. Berdova-G.;S. Mikhalap;J. Van Lint;S. P. Sidorenko
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作者：
L. Kovalevska;O. Yurchenko;L. Shlapatska;G. G. Berdova-G.;S. Mikhalap;J. Van Lint;S. P. Sidorenko
目的研究PKD2在反应性淋巴结和淋巴组织肿瘤中的表达、自身磷酸化和定位。材料和方法识别 PKD1/2 或 PKD2 和自磷酸化 PKD1/2 的特异性抗体用于扁桃体、反应性淋巴结、非霍奇金淋巴瘤 (NHL) 和霍奇金淋巴瘤 (HL) 肿瘤样本的免疫组织化学和生化研究。结果 PKD1和PKD2表达的免疫组织化学和生化分析显示，NHL和HL患者的扁桃体、反应性淋巴结和肿瘤组织中都有PKD2表达。此外，我们无法揭示所研究的淋巴组织中的 PKD1 表达。在扁桃体和反应性淋巴结中，在 T 细胞和 B 细胞区检测到 PKD2 表达，在淋巴滤泡的生发中心中检测到最高水平，在生发中心的亮区中自身磷酸化水平最高。我们发现低水平的 PKD2 表达和自身磷酸化是套细胞淋巴瘤、伯基特淋巴瘤以及 50% 的 CLL/小淋巴细胞淋巴瘤的特征。生发中心来源且具有激活的 B 细胞表型的淋巴瘤细胞（弥漫性大 B 细胞淋巴瘤，HL）和间变性大细胞淋巴瘤表现出高水平的 PKD2 表达和自磷酸化。结论 肿瘤细胞中 PKD2 表达和自磷酸化的水平对应于该激酶在其正常类似物中的表达模式，并且对应于细胞分化和激活的水平。
AIM To study the PKD2 expression, autophosphorylation and localization in reactive lymph nodes and tumors of lymphoid tissues. MATERIALS AND METHODS Specific antibodies, which recognize PKD1/2 or PKD2 and autophosphorylated PKD1/2, were used for immunohistochemical and biochemical studies of tonsils, reactive lymph nodes, tumor samples of non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL). RESULTS Immunohistochemical and biochemical analysis of PKD1 and PKD2 expression showed PKD2 expression in tonsils, reactive lymph nodes and tumor tissues from patients with NHL and HL. Furthermore, we were not able to reveal PKD1 expression in studied lymphoid tissues. In tonsils and reactive lymph nodes the PKD2 expression was detected in T and B cell zones with highest level in germinal centers of lymphoid follicles and the maximum level of autophosphorylation in the light zones of the germinal centers. We found that low level of PKD2 expression and autophosphorylation was characteristic feature for mantle cell lymphomas, Burkitt's lymphomas, and in 50% of CLL/small lymphocytic lymphomas. Lymphoma cells of germinal center origin and with activated B cell phenotype (diffuse large B cell lymphomas, HL) and anaplastic large cells lymphoma demonstrated the high level of PKD2 expression and autophosphorylation. CONCLUSIONS The level of PKD2 expression and autophosphorylation in neoplastic cells corresponds to the expression pattern of this kinase in their normal analogs, and to the level of cell differentiation and activation.