Marked Difference in the Conformational Transition of DNA Caused by Propanol Isomer
Marked Difference in the Conformational Transition of DNA Caused by Propanol Isomer
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DOI：
10.3390/polym12071607
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发表时间：
2020-07-01
期刊：
影响因子：
5
通讯作者：
Yoshikawa, Kenichi
中科院分区：
文献类型：
--
作者：
Ma, Yue;Yoshikawa, Yuko;Yoshikawa, Kenichi
我们通过单分子观察测量了在不同浓度的1-和2-丙醇下DNA分子（λ噬菌体DNA，48 kbp）的高阶结构的变化。已知与1-丙醇相反，2-丙醇通常适用于从活细胞/器官分离基因组DNA的程序。在本研究中，人们发现，随着1-丙醇浓度的增加，DNA表现出从一个细长的线圈折叠小球，然后到一个未折叠的状态折返构象转变。另一方面，对于2-丙醇，DNA表现出单调收缩成紧凑状态。在直流（DC）电势下的拉伸实验揭示了被1-和2-丙醇中间收缩的单个DNA分子表现出链内相分离，即，细长部分和紧凑部分共存。1-丙醇的特征效应引起的重入过渡的富水纳米团簇的产生方面的争论。
We measured the changes in the higher-order structure of DNA molecules (lambda phage DNA, 48 kbp) at different concentrations of 1- and 2-propanol through single-molecular observation. It is known that 2-propanol is usually adapted for the procedure to isolate genomic DNA from living cells/organs in contrast to 1-propanol. In the present study, it was found that with an increasing concentration of 1-propanol, DNA exhibits reentrant conformational transitions from an elongated coil to a folded globule, and then to an unfolded state. On the other hand, with 2-propanol, DNA exhibits monotonous shrinkage into a compact state. Stretching experiments under direct current (DC) electrical potential revealed that single DNA molecules intermediately shrunk by 1- and 2-propanol exhibit intrachain phase segregation, i.e., coexistence of elongated and compact parts. The characteristic effect of 1-propanol causing the reentrant transition is argued in terms of the generation of water-rich nanoclusters.