Dynamic Cultivation of Human Mesenchymal Stem Cells in a Rotating Bed Bioreactor System Based on the Z®RP Platform
Dynamic Cultivation of Human Mesenchymal Stem Cells in a Rotating Bed Bioreactor System Based on the Z®RP Platform
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DOI：
10.1002/btpr.258
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发表时间：
2009-11-01
影响因子：
2.9
通讯作者：
Kasper, Cornelia
中科院分区：
文献类型：
--
作者：
Diederichs, Solvig;Roeker, Stefanie;Kasper, Cornelia
由于大面积骨缺损的再生受到数量限制和感染风险的限制，生物人工骨替代物的开发具有重要意义。为了获得三维功能性组织样移植物，由于细胞密度、营养和氧气支持的限制，静态培养是不适宜的。在生物反应器系统中的动态培养可以克服这些限制，并且还提供了控制关于pH、氧含量和温度的环境的可能性。在这项研究中，一个三维骨结构工程使用动态生物反应器技术。在称为Sponceram的大孔二氧化锆基陶瓷盘上培养人脂肪组织来源的间充质干细胞。此外，使用羟基磷灰石涂覆的Sponceram。在动态条件下培养细胞，并与静态培养的细胞进行比较。成骨细胞的分化由成骨补充剂启动。通过测定葡萄糖和乳酸浓度，比较静态和动态培养期间的细胞增殖。分化过程进行了分析，确定AP表达和使用不同的特异性染色方法。我们的研究结果表明，在动态条件下，在生物反应器系统中的增殖率高得多相比，静态培养葡萄糖消耗和乳酸盐的生产。支架上的细胞密度表明，与羟基磷灰石涂覆的Sponceram（R）相比，天然Sponceram（R）上的增殖更高。通过这项研究，我们提出了一种极好的方法来增强细胞增殖和骨谱系特异性生长的组织样结构，包括纤维（胶原）和球状（矿物质）细胞外成分。(C)2009年美国化学工程师学会生物技术。程序，25日：1762-1771 2009年
Because the regeneration of large bone defects is limited by quantitative restrictions and risks of infections, the development of bioartificial bone substitutes is of great importance. To obtain a three-dimensional functional tissue-like graft, static cultivation is inexpedient due to limitations in cell density, nutrition and oxygen support. Dynamic cultivation in a bioreactor system can overcome these restrictions and furthermore provide the possibility to control the environment with regard to pH, oxygen content, and temperature. In this study, a three-dimensional bone construct was engineered by the use of dynamic bioreactor technology. Human adipose tissue derived mesenchymal stein cells were cultivated on a macroporous zirconium dioxide based ceramic disc called Sponceram (R). Furthermore, hydroxyapatite coated Sponceram (R) was used. The cells were cultivated under dynamic conditions and compared with statically cultivated cells. The differentiation into osteoblasts was initiated by osteogenic supplements. Cellular proliferation during static and dynamic cultivation was compared measuring glucose and lactate concentration. The differentiation process was analysed determining AP-expression and using different specific staining methods. Our results demonstrate much higher proliferation rates during dynamic conditions in the bioreactor-system compared to static cultivation measured by glucose consumption and lactate production. Cell densities on the scaffolds indicated higher proliferation on native Sponceram (R) compared to hydroxyapatite coated Sponceram (R). With this study, we Present an excellent method to enhance cellular proliferation and bone lineage specific growth of tissue like structures comprising fibrous (collagen) and globular (mineral) extracellular components. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1762-1771 2009