Localization of transferrin and transferrin receptors in rat testes.
Localization of transferrin and transferrin receptors in rat testes.
复制标题
大鼠睾丸中转铁蛋白和转铁蛋白受体的定位。
DOI：
10.1095/biolreprod31.1.195
复制
发表时间：
1984
影响因子：
3.6
通讯作者：
Griswold,MD
中科院分区：
文献类型：
--
作者：
Sylvester,SR;Griswold,MD
One of the major proteins secreted by rat Sertoli cells in culture is a transferrin-like protein (Skinner and Griswold, 1980). The purpose of this study was to quantitate the amount of testicular transferrin in fluids isolated from the testis by the use of a radioimmunoassay and to determine the location of transferrin and transferrin receptors in the testis by indirect immunofluorescence. Seminiferous tubule fluid, rete testis fluid, and testicular lymph were collected from rat testes and were found to contain 141 pg. 47 pg and 3.7 mg transferrin per ml of fluid, respectively. Serum was found to contain 3.7 mg/mI transferrin. Paraffin sections of rat testis were incubated with rabbit anti-rat transferrin, biotinylated goat anti-rabbit and fluorescein-conjugated avidin. Immunoreactive transferrin was thus localized on the proacrosome and nuclear cap of developing spermatids. Late spermatids showed transferrin over the entire region of the head but mature testicular spermatozoa exhibited little fluorescence. The interstitital tissue between seminiferous tubules fluoresced brightly, indicating a large amount of transferrin in this area. By pretreating sections with rat transferrin, the receptor for the protein was localized on and in spermatocytes and early round spermatids. Dividing germ cells were brightly fluorescent.
