A monoclonal antibody specific for rat IL-18BP and its application in determining serum IL-18BP
A monoclonal antibody specific for rat IL-18BP and its application in determining serum IL-18BP
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DOI：
10.1016/s0165-2478(02)00213-4
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发表时间：
2003-01-02
影响因子：
4.4
通讯作者：
Fuchs, S
中科院分区：
文献类型：
--
作者：
Maiti, PK;Im, SH;Fuchs, S
IL-18结合蛋白（IL-18 BP）是IL-18的可溶性抑制性受体，IL-18是在IL-12驱动的Th 1细胞分化中起主要作用的促炎细胞因子。IL-18 BP被认为在IL-18介导的免疫应答中发挥重要的调节功能，尽管其在各种疾病中的免疫调节中的确切作用还不清楚。在本研究中，我们已经产生并表征了小鼠单克隆抗体（2A 6 H11），特异性大鼠IL-18 BP。其结合表位在IL-18 BP上的氨基酸29-60内，并且其不干扰IL-18 BP的功能。使用该单克隆抗体，我们已经开发了用于测定IL-18 BP水平的ELISA。实验性自身免疫性重症肌无力（EAMG）大鼠血清IL-18 BP水平升高，与健康大鼠相比。因此，这种单克隆抗体可以作为一个有价值的工具，以阐明IL-18 BP在EAMG和其他大鼠模型的自身免疫性疾病的作用。(C)2002 Elsevier Science B. V.保留所有权利。
IL-18 binding protein (IL-18BP) is a soluble inhibitory receptor for IL-18, a proinflammatory cytokine that plays a major role in IL-12-driven Th1 cell differentiation. IL-18BP is presumed to play a vital regulatory function in IL-18-mediated immune responses, although its precise role in immunomodulation in various diseases is not well understood. In the present study, we have generated and characterized a mouse monoclonal antibody (2A6H11), specific for rat IL-18BP. Its binding epitope is within amino acids 29-60 on IL-18BP and it does not interfere with the function of IL-18BP. Using this monoclonal antibody, we have developed an ELISA for determining IL-18BP levels. We observed elevated levels of IL-18BP in sera of rats with experimental autoimmune myasthenia gravis (EAMG), in comparison with the levels in healthy rats. Thus, this monoclonal antibody can serve as a valuable tool to elucidate the role of IL-18BP in EAMG and in other rat models for autoimmune diseases. (C) 2002 Elsevier Science B.V. All rights reserved.