Preservation of Neisseria gonorrhoeae by the gelatin-disc method.
Preservation of Neisseria gonorrhoeae by the gelatin-disc method.
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明胶圆盘法保存淋病奈瑟菌。
DOI：
10.1136/sti.55.2.90
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
Kanagawa Prefectural
中科院分区：
文献类型：
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作者：
S. Yamai;Y. Obara;T. Nikkawa;Y. Shimoda;Y. Miyamoto;Kanagawa Prefectural
淋病奈瑟菌的研究很难进行，因为该生物体的体外存活率很低。为了解决这个问题，我们试图用明胶圆盘法保存生物体。本文研究了八个N.淋病被跟踪了三年。这些研究证明，活细胞仅减少1/10，保存效果令人满意。另一项试验表明，这种生物在零下20摄氏度冷冻后存活了6个多月。保存3年后，菌落类型、对家兔和豚鼠红细胞的凝集反应以及对青霉素、氯霉素、四环素、卡那霉素和链霉素的敏感性均无变化。
Studies of Neisseria gonorrhoeae are difficult to perform because of the organism's poor survival in vitro. To solve this problem we tried to preserve the organism by a gelatin-disc method. The rate of survival and changes of variations in some biochemical properties of eight strains of N. gonorrhoeae were followed for three years. These studies proved that preservation was satisfactory with only a 1/10 reduction of the living cells. Another trial showed that the organism survived for over six months after being frozen at -20 degrees C. The colonial types, agglutination against red cells from rabbit and guinea pig, and antibiotic susceptibility to penicillin, chloramphenicol, tetracycline, kanamycin, and streptomycin did not change after three years' preservation.