Microarray analyses of gene expression during adventitious root development in Pinus contorta 1[w]
Microarray analyses of gene expression during adventitious root development in Pinus contorta 1[w]
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DOI：
10.1104/pp.103.032235
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发表时间：
2004-07-01
期刊：
影响因子：
7.4
通讯作者：
von Arnold, S
中科院分区：
文献类型：
--
作者：
Brinker, M;van Zyl, L;von Arnold, S
为了研究不定根发育过程中的基因表达模式，利用生长素吲哚-3-丁酸脉冲处理的弯曲松（Pinus contorta）下胚轴的RNA，在不同的根发育时间点进行杂交，并将其与含有2178个来自油松（Pinus taeda） cdna的芯片进行杂交。在根系发育观察期间，220个基因的转录水平发生了显著变化。在根起始阶段，参与细胞复制和细胞壁弱化的基因以及编码PINHEAD/ zwille样蛋白的转录物被上调，而与生长素运输、光合作用和细胞壁合成相关的基因被下调。此外，水分胁迫相关基因的转录丰度也发生了变化。在根分生组织形成阶段，与生长素运输、生长素应答转录和细胞壁合成有关的基因和编码b盒锌指样蛋白的基因的转录丰度增加，而与细胞壁削弱有关的编码蛋白的转录丰度减少。在根分生组织形成和根形成阶段，水分胁迫相关基因转录丰度的变化表明植物根已具备了水分运输功能。与此同时，与生长素运输相关的基因被上调，而与细胞壁修饰相关的基因被下调。最后，在根伸长阶段，编码参与细胞复制和胁迫的蛋白的转录本出现下调。根据观察到的转录物丰度变化，我们提出了生长素诱导根发育过程中各种生理过程相对重要性的假设。
In order to investigate the gene expression pattern during adventitious root development, RNA of Pinus contorta hypocotyls, pulse-treated with the auxin indole-3-butyric acid and harvested at distinct developmental time points of root development, was hybridized to microarrays containing 2,178 cDNAs from Pinus taeda. Over the period of observation of root development, the transcript levels of 220 genes changed significantly. During the root initiation phase, genes involved in cell replication and cell wall weakening and a transcript encoding a PINHEAD/ZWILLE-like protein were up-regulated, while genes related to auxin transport, photosynthesis, and cell wall synthesis were down-regulated. In addition, there were changes in transcript abundance of genes related to water stress. During the root meristem formation phase the transcript abundances of genes involved in auxin transport, auxin responsive transcription, and cell wall synthesis, and of a gene encoding a B-box zinc finger-like protein, increased, while those encoding proteins involved in cell wall weakening decreased. Changes of transcript abundance of genes related to water stress during the root meristem formation and root formation phase indicate that the plant roots had become functional in water transport. Simultaneously, genes involved in auxin transport were up-regulated, while genes related to cell wall modification were down-regulated. Finally, during the root elongation phase down-regulation of transcripts encoding proteins involved in cell replication and stress occurred. Based on the observed changes in transcript abundances, we suggest hypotheses about the relative importance of various physiological processes during the auxin-induced development of roots in P. contorta.