The construction of a genetic linkage map of red raspberry (Rubus idaeus subsp idaeus) based on AFLPs, genomic-SSR and EST-SSR markers
The construction of a genetic linkage map of red raspberry (Rubus idaeus subsp idaeus) based on AFLPs, genomic-SSR and EST-SSR markers
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DOI：
10.1007/s00122-004-1687-8
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发表时间：
2004-08-01
影响因子：
5.4
通讯作者：
Powell, W
中科院分区：
文献类型：
--
作者：
Graham, J;Smith, K;Powell, W
树莓的育种是耗时的，因为这种多年生水果作物的高度杂合性，加上相对较长的幼年期。利用遗传连锁图谱可以提高树莓育种的速度和精度，从而有利于开发多基因性状的诊断标记和鉴定控制复杂表型的基因。红树莓(Rubus Idaeus)的遗传连锁图谱(789 CM)是由两个表型不同的品种杂交而成的：最近的欧洲品种Glen Moy和较老的北美品种Latham。SSR标记来源于Glen Moy的基因组文库和cDNA文库。这些SSR与AFLP标记一起被用来创建连锁图谱。为了检验遗传连锁图谱在QTL分析中的实用性，收集了基于易记分表型性状的形态数据。在两个不同的环境中，量化了甘蔗刺度的分离，以及密度和从母株扩散的根部吸盘性状。利用MapQTL分析了这些性状与作图标记的显著连锁关系，发现这些性状位于脊椎连锁群2上，密度和直径位于连锁群8上。共显性标记的可用性允许计算两个品种的杂合度。
Breeding in raspberry is time-consuming due to the highly heterozygous nature of this perennial fruit crop, coupled with relatively long periods of juvenility. The speed and precision of raspberry breeding can be improved by genetic linkage maps, thus facilitating the development of diagnostic markers for polygenic traits and the identification of genes controlling complex phenotypes. A genetic linkage map (789 cM) of the red raspberry Rubus idaeus has been constructed from a cross between two phenotypically different cultivars; the recent European cultivar Glen Moy and the older North American cultivar Latham. SSR markers were developed from both genomic and cDNA libraries from Glen Moy. These SSRs, together with AFLP markers, were utilised to create a linkage map. In order to test the utility of the genetic linkage map for QTL analysis, morphological data based on easily scoreable phenotypic traits were collected. The segregation of cane spininess, and the root sucker traits of density and spread from the mother plant, was quantified in two different environments. These traits were analysed for significant linkages to mapped markers using MapQTL and were found to be located on linkage group 2 for spines and group 8 for density and diameter. The availability of co-dominant markers allowed heterozygosities to be calculated for both cultivars.