Induction of zonal and egg plasma membrane blocks to sperm penetration in mouse eggs with cortical granule exudate.
Induction of zonal and egg plasma membrane blocks to sperm penetration in mouse eggs with cortical granule exudate.
复制标题
带状和卵质膜的诱导阻止精子穿透具有皮质颗粒渗出物的小鼠卵子。
DOI：
10.1095/biolreprod17.3.350
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发表时间：
1977
影响因子：
3.6
通讯作者：
Mitsuma Hamada
中科院分区：
文献类型：
--
作者：
Don P. Wolf;Mitsuma Hamada
The contents of cortical granules (cortical granule exudate = CGE) were recovered from zona-free mouse eggs inseminated in vitro with capacitated epididymal spermatozoa. Preincubation of cumulus-free, zona-intact eggs in CGE led to reduced penetration levels upon insemination. This reduction was CGE concentration- and time-dependent and was susceptible to inhibition by soybean trypsin inhibitor and by p-aminobenzamidine. Control experiments eliminated active contributions from the sperm suspension used to elicit granule release. CGE was also active in reducing penetration of zona-free eggs. These results indicate that cortical granule contents are capable of modulating sperm penetration of mouse eggs at both the zona pellucida and at the egg plasma membrane.
