Basophils contribute to TH2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells
Basophils contribute to TH2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells
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DOI：
10.1038/ni.1737
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发表时间：
2009-07-01
影响因子：
30.5
通讯作者：
Nakanishi, Kenji
中科院分区：
文献类型：
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作者：
Yoshimoto, Tomohiro;Yasuda, Koubun;Nakanishi, Kenji
嗜碱性粒细胞表达主要组织相容性复合物II类，CD 80和CD 86，并在各种条件下产生白细胞介素4（IL-4）。在这里，我们表明，当孵育与IL-3和抗原或抗原和免疫球蛋白E（IgE）的复合物，嗜碱性粒细胞内化，加工和抗原作为复合物的肽和主要组织相容性复合物II类，并产生IL-4。将卵清蛋白脉冲的嗜碱性粒细胞静脉注射到幼稚小鼠体内，“优先”诱导幼稚卵清蛋白特异性CD 4（+）T细胞发育为2型T辅助细胞（T（H）2）。以这种方式免疫的小鼠，当通过静脉内施用卵清蛋白进行攻击时，迅速产生卵清蛋白特异性IgG 1和IgE。最后，静脉注射IgE复合物仅在存在内源性嗜碱性粒细胞的情况下快速诱导T（H）2细胞，这表明嗜碱性粒细胞是有效的抗原呈递细胞，其通过捕获IgE复合物“优先”增强T（H）2-IgE应答。
Basophils express major histocompatibility complex class II, CD80 and CD86 and produce interleukin 4 (IL-4) in various conditions. Here we show that when incubated with IL-3 and antigen or complexes of antigen and immunoglobulin E (IgE), basophils internalized, processed and presented antigen as complexes of peptide and major histocompatibility complex class II and produced IL-4. Intravenous administration of ovalbumin-pulsed basophils into naive mice 'preferentially' induced the development of naive ovalbumin-specific CD4(+) T cells into T helper type 2 (T(H)2) cells. Mice immunized in this way, when challenged by intravenous administration of ovalbumin, promptly produced ovalbumin-specific IgG1 and IgE. Finally, intravenous administration of IgE complexes rapidly induced T(H)2 cells only in the presence of endogenous basophils, which suggests that basophils are potent antigen-presenting cells that 'preferentially' augment T(H)2-IgE responses by capturing IgE complex.