Induction of Deformed Pollen Tube Tips and Their Morphological Characteristics in Self-incompatible Japanese Pear
Induction of Deformed Pollen Tube Tips and Their Morphological Characteristics in Self-incompatible Japanese Pear
复制标题
自交不亲和梨花粉管尖端变形的诱导及其形态特征
DOI：
10.2503/jjshs.60.257
复制
发表时间：
1991
影响因子：
--
通讯作者：
J. Matsushima
中科院分区：
文献类型：
--
作者：
S. Hiratsuka;Yohko Kitoh;J. Matsushima
日本梨，梨，花粉不兼容和兼容授粉后，在体内研究的花粉管尖端的畸变;和在体外研究的化学添加剂的影响，生长medium.Incompatible和兼容的花粉/柱头风格组合导致的花粉管与变形的尖端，主要是检测到肿胀。不兼容的组合开发了大约两倍的肿胀提示。在离体条件下，阿坝、OE、PEG、H_3B_（03）和FC等处理均能增加异常花粉管顶端的数量。这些管的尖端表面是相当不规则的，除了在H3 BO 3的情况下，这似乎是类似于不兼容的风格中观察到的那些变形的提示。硼酸引起的规则细沟沿着管轴，但氯化钙对变形尖端的形成没有影响，管表面结构是相对光滑的。因此，生长抑制剂倾向于诱导类似于在不相容花柱中生长的花粉管上观察到的那些的不规则性在花粉管表面上，而生长促进剂不诱导这样的症状。顶端形态的相似性可能表明，在不相容的风格中生长的花粉管中的细胞壁合成被抑制剂或水分胁迫改变，随后在柱头表面和/或在传输组织中发生特异性识别反应。这种变化导致管生长停止。
Aberrations in pollen tube tips of Japanese pear, Pyrus serotina, in vivo studies following pollen-incompatible and -compatible pollinations; and in vitro studies on the effects of chemical additives to the growth medium were investigated.Incompatible and compatible pollen/stigma-style combinations resulted in pollen tubes with deformed tips which were mainly detected as a swelling. The incompatible combinations developed approximately twice as many swollen tips. Their surface structure revealed irregular features such as concave and unusual furrows, whereas a smooth surface was observed in normally growing tips.In in vitro trials, ABA, ovarian extract (OE), polyethylene glycol (PEG), H3B03 and fusicoccin (FC) increased the number of abnormal pollen tube tips. The tip surfaces of these tubes were considerably irregular, except in the case of H3BO3, which seemed to be similar to those deformed tips observed in incompatible styles. Boric acid caused regular fine furrows along the tube axis but CaCl2 had no effect on the deformed tip formation; the tube surface structure was relatively smooth. Thus, the growth inhibitors tend to induce irregularities on pollen tube surface similar to those observed on pollen tubes growing in an incompatible style, whereas growth promoters did not induce such symptoms. The similarities in tip morphology may indicate that cell wall synthesis in pollen tubes growing in incompatible styles is altered by inhibitors or water stress following a specific recognition reaction on the stigmatic surface and/or in the transmitting tissue. This alteration causes cessation of tube growth.