High HPV16 E6 viral load in the oral cavity is associated with an increased number of bacteria: A preliminary study
High HPV16 E6 viral load in the oral cavity is associated with an increased number of bacteria: A preliminary study
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DOI：
10.3892/br.2017.1025
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发表时间：
2018-01-01
影响因子：
2.3
通讯作者：
Takechi, Masaaki
中科院分区：
文献类型：
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作者：
Shigeishi, Hideo;Sugiyama, Masaru;Takechi, Masaaki
在以前的一项研究中，本研究小组报告说，在口腔冲洗样本中，男性的人乳头瘤病毒（HPV）16的患病率明显高于女性。本研究的目的是探讨HPV 16病毒载量与临床因素之间的关系，包括剩余牙齿，义齿使用和口腔细菌数量。对2016年11月至2017年8月期间在广岛大学医院（日本广岛）口腔颌面重建外科就诊的共计124例患者（48例男性和76例女性;平均年龄61.6岁;年龄范围20-97岁）进行了分析。没有一个病人有口腔癌或癌前病变的证据，包括上皮发育不良和白斑。采用定量聚合酶链反应（qPCR）分析来检查HPV 16病毒拷贝数。此外，使用介电电泳阻抗测量方法测定口腔细菌的数量。从124例受试者中的30例中获得的样本的HPV 16低于qPCR结果的检测限，因此在其余94例患者中检查了HPV 16病毒拷贝数与临床参数的相关性。HPV 16 E6 DNA拷贝数平均为1.65 +/- 3.47拷贝/细胞（范围0.07-25.3拷贝/细胞），口腔细菌计数高[>= 10（6.5）菌落形成单位（CFU）/ml]的受试者的HPV 16 E6 DNA拷贝数显著高于口腔细菌计数低（
In a previous study, the present research group reported that males had a significantly higher prevalence of human papillomavirus (HPV) 16 than females in oral rinse samples. The objective of the present study was to examine the relationship between HPV16 viral load and clinical factors, including remaining teeth, denture use and numbers of oral bacteria. A total of 124 patients (48 males and 76 females; mean age, 61.6 years; age range, 20-97 years) who visited the Department of Oral and Maxillofacial Reconstructive Surgery of Hiroshima University Hospital (Hiroshima, Japan) between November 2016 and August 2017 were analyzed. None of the patients had evidence of oral cancer or pre-malignant lesions, including epithelial dysplasia and leukoplakia. Quantitative polymerase chain reaction (qPCR) analysis was employed to examine the number of HPV16 viral copies. Furthermore, the number of oral bacteria was determined using the dielectrophoretic impedance measurement method. HPV16 was below the limit of detection in qPCR findings for samples obtained from 30 of the 124 subjects, thus the association of HPV16 viral copy number with clinical parameters was examined in the remaining 94 patients. The average number of HPV16 E6 DNA copies was 1.65 +/- 3.47 copies/cell (range, 0.07-25.3 copies/cell) and was significantly higher in subjects with a high oral bacteria count [>= 10(6.5) colony forming unit (CFU)/ml] than in those with a low count (