3-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion
3-Daidzein sulfonate sodium inhibits neuronal apoptosis induced by cerebral ischemia-reperfusion
复制标题
3'-大豆苷元磺酸钠抑制脑缺血再灌注诱导的神经细胞凋亡
DOI：
10.3892/ijmm.2017.2915
复制
发表时间：
2017-04-01
影响因子：
5.4
通讯作者：
Li, Dongliang
中科院分区：
文献类型：
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作者：
Liu, Ruizhen;Zhong, Xinming;Li, Dongliang
本研究旨在观察3-大豆苷元磺酸钠（DSS）对脑缺血再灌注损伤的影响，并探讨DSS诱导神经细胞凋亡的机制。使用大脑中动脉闭塞在雄性Sprague-Dawley大鼠中诱导局灶性缺血。根据假手术或真实的脑缺血分为5组，分别给予不同剂量的DSS（0.5、1.0和2.0 mg/kg）或生理盐水（模型组），于脑缺血前注射。缺血2 h再灌注24 h后，采用Longa评分标准进行神经功能缺损评分。采用氯化三苯基四氮唑染色技术测量梗死体积。用伊文思蓝（EB）含量测定血脑屏障（BBB）通透性，并用电镜观察超微结构变化。采用免疫组化和western blot方法检测Bcl-2、Bax和caspase-3的表达。DSS各剂量组大鼠的神经功能缺损程度均显著降低（P
This study aimed to observe the effects of 3-daidzein sulfonate sodium (DSS) on ischemia-reperfusion-induced brain injury and to analyze the mechanisms responsible for neuronal apoptosis. Focal ischemias were induced in male Sprague-Dawley rats using middle cerebral artery occlusion. The rats were divided into 5 groups based on sham surgery or real occlusion, and treatment with different doses of DSS (0.5, 1.0 and 2.0 mg/kg) or normal saline (model group), injected preoperatively into the rats with cerebral occlusion. After 2 h of ischemia and 24 h of reperfusion, neurological deficit scores were evaluated using the Longa grade point standard. The infarct volume was measured using a triphenyl tetrazolium chloride staining technique. Blood-brain barrier (BBB) permeability was measured using the Evans blue (EB) content of brain tissues, while electron microscopy was used to observe ultrastructural changes. The expression levels of Bcl-2, Bax and caspase-3 were detected by an immunohistochemical method and western blot analysis. The neurological deficit in rats pre-treated with DSS at all doses decreased significantly (P