Identification and characterization of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii
Identification and characterization of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii
复制标题
DOI：
10.1128/jb.188.5.2014-2019.2006
复制
发表时间：
2006-03-01
影响因子：
3.2
通讯作者：
Wakagi, T
中科院分区：
文献类型：
--
作者：
Nishimasu, H;Fushinobu, S;Wakagi, T
作为葡萄糖磷酸化酶的一个新成员，从嗜热的古细菌Sulfolobus tokodaii中纯化、鉴定和表征了atp依赖性己糖激酶。我们的研究结果表明，该酶不同于其他已知的酶在初级结构和广泛的底物特异性磷酰供体和受体。
As a new member of the glucose-phosphorylating enzymes, the ATP-dependent hexokinase from the hyperthermophilic crenarchaeon Sulfolobus tokodaii was purified, identified, and characterized. Our results revealed that the enzyme differs from other known enzymes in primary structure and its broad substrate specificity for both phosphoryl donors and acceptors.