Influence of nitrogen and phosphorus additions on N2-fixation activity, abundance, and composition of diazotrophic communities in a Chinese fir plantation.
Influence of nitrogen and phosphorus additions on N2-fixation activity, abundance, and composition of diazotrophic communities in a Chinese fir plantation.
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DOI：
10.1016/j.scitotenv.2017.10.064
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发表时间：
2017-11
期刊：
影响因子：
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通讯作者：
Qing Wang;Jianlei Wang;Yuanzheng Li;Diwen Chen;Junhua Ao;Wenling Zhou;Dachun Shen;Qi-wei Li
中科院分区：
文献类型：
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作者：
Qing Wang;Jianlei Wang;Yuanzheng Li;Diwen Chen;Junhua Ao;Wenling Zhou;Dachun Shen;Qi-wei Li
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Although biological nitrogen (N) fixation (BNF) is an important N input process in subtropical forest ecosystems, how the diazotrophic communities related to this process respond to N and phosphorus (P) inputs is largely unknown. We investigated the effects of exogenous N and/or P inputs on N2-fixation activity, diazotrophic abundance and community composition using a continuous application of fertilizers over 5 years experiment in a Chinese fir plantation. The fertilization regimes included control (CK), P treatment (P), low N addition treatment (N1), high N addition treatment (N2), low N and P addition treatment (N1P) and high N with P addition treatment (N2P). N2-fixation activity was determined using the acetylene reduction assay (ARA). Quantitative PCR and Illumina Miseq sequencing ofnifHgene were performed to analyze diazotrophic abundance and community composition, respectively. Our results showed that P addition increased N2-fixation activity andnifHgene abundance by 189.07 n mol C2H4and 1.02 × 107copies g− 1dry soil, respectively, while were reduced by 1.19 n mol C2H4and 2.04 × 106copies g− 1dry soil when N was added. The application of P with low N (N1P) effectively alleviated the inhibitory effect of N input on N2-fixation activity. N-related treatments resulted in significant decreases in operational taxonomic unit (OTU) richness and shifts in diazotrophic community structure. N2-fixation activity andnifHgene abundance were strongly and positively correlated with soil pH and negatively correlated with mineral N (NH4+-N and NO3−-N) contents, while mineral N concentrations rather than soil pH appeared to be the main factor altering diazotrophic community structure. These results revealed that P addition played a positive role in regulating biological nitrogen fixation in subtropical forest ecosystems.