Effect of temperature on Wolbachia density and impact on cytoplasmic incompatibility
Effect of temperature on Wolbachia density and impact on cytoplasmic incompatibility
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DOI：
10.1017/s0031182005008723
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发表时间：
2006-01-01
期刊：
影响因子：
2.4
通讯作者：
Vavre, F
中科院分区：
文献类型：
--
作者：
Mouton, L;Henri, H;Vavre, F
宿主-共生体关联的结果和进化取决于环境限制，但反应很难预测，因为它们是由宿主、寄生虫和环境之间复杂的相互作用产生的。当多种寄生虫基因型（对环境变化可能有不同的反应）共存于一个宿主体内时，情况可能会更加复杂。在本文中，我们研究了宿主发育过程中温度（14 至 26 摄氏度）对共存于黄蜂细毛虫杂瘤内的 3 种细胞内细菌沃尔巴克氏体密度的影响。在这个物种中，沃尔巴克氏体会引起细胞质不相容，这是一种精卵不相容，使其能够在宿主群体中传播和持续存在。使用实时定量 PCR，我们发现 (i) 沃尔巴克氏体密度具有温度特异性，在 26 摄氏度时最高； (ii) 3个沃尔巴克氏体菌株的丰度顺序不随温度变化而变化； (iii) 细菌密度对温度的响应发生在单代昆虫内，即从卵到成虫的发育期间； (iv) 在该物种中，沃尔巴克氏体密度与温度相关的变化不会影响细胞质不相容性。
The outcome and the evolution of host-symbiont associations depend on environmental constraints, but responses are difficult to predict since they arise from a complex interaction between the host, the parasite and the environment. The situation can be even more complex when multiple parasite genotypes, with potentially different responses to environmental changes, coexist within a single host. In this paper, we investigated the effect of the temperature (from 14 to 26 degrees C) during the host development on the density of 3 strains of the intracellular bacterium Wolbachia that coexist within the wasp Leptopilina heterotoma. In this species, Wolbachia induces cytoplasmic incompatibility, a sperm-egg incompatibility that allows it to spread and persist in host populations. Using real-time quantitative PCR we found that (i) Wolbachia density is temperature-specific and highest at 26 degrees C; (ii) the order of the abundance of the 3 Wolbachia strains does not vary with temperature changes; (iii) the response of bacterial density to temperature occurs within a single insect generation, during the egg-to-adult developmental period; (iv) in this species, temperature-related changes in Wolbachia density do not influence cytoplasmic incompatibility.