Pistil induction by hormones from callus of Oryza sativa in vitro
Pistil induction by hormones from callus of Oryza sativa in vitro
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DOI：
10.1007/s00299-004-0864-1
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发表时间：
2004-12-01
影响因子：
6.2
通讯作者：
Zhang, XS
中科院分区：
文献类型：
--
作者：
An, YR;Li, XG;Zhang, XS
本研究报道了水稻雌蕊外植体愈伤组织诱导花器官雌蕊的成功形成。在花器官的诱导方面，以不同的胚、外稃、内稃和雌蕊为外植体，用不同的N-6-苄基腺嘌呤和2，4-二氯苯氧乙酸（2，4-D）组合诱导愈伤组织。雌蕊和幼胚外植体愈伤组织形成频率高。将雌蕊愈伤组织转移到含玉米素和2，4-D的培养基上，诱导出花器官。雌蕊状器官的形态特征与植物体内形成的雌蕊状器官相似，但差异不大。进一步的组织学研究表明，离体雌蕊的子房内含有胚珠。此外，雌蕊特异性基因OsMADS 3用作雌蕊身份的分子标记，在雌蕊样器官中表达，因为它在植物花的雌蕊中。
This study describes the successful formation of floral organ pistil from the callus of pistil explants of Oryza sativa L. For induction of floral organs, different explants-including young embryo, lemma, palea and pistil-were used 6 for callus induction with different combinations of N-6-benzyladenine and 2,4-dichlorophenoxyacetic acid (2,4-D). High frequencies of callus formation from pistil and young embryo explants were achieved. Floral organs were induced after calli from pistils were transferred to medium containing both zeatin and 2,4-D. The morphological characteristics of the pistil-like organs are very similar to those formed in planta though with minor differences. Further histological study revealed that the in vitro pistil contains an ovule within its ovary. Furthermore, a pistil-specific gene, OsMADS3 used as a molecular marker for pistil identity, was expressed in the pistil-like organs as it was in pistils in the flower of the plant.