Host membrane proteins involved in the replication of tobamovirus RNA
Host membrane proteins involved in the replication of tobamovirus RNA
复制标题
DOI：
10.1016/j.coviro.2012.09.011
复制
发表时间：
2012-12-01
影响因子：
5.9
通讯作者：
Ishikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Ishibashi, Kazuhiro;Miyashita, Shuhei;Ishikawa, Masayuki
真核正链RNA病毒在由病毒复制蛋白和负链RNA模板组成的膜结合复制复合体中复制自己的基因组。这些复制蛋白被编程为仅在复制复合体中表现出RNA聚合酶和其他复制相关活性，以避免诱导双链RNA介导的宿主防御。宿主膜成分(如蛋白质和脂类)在复制蛋白的激活过程中应发挥重要作用。两个宿主膜蛋白是复制复合体的组成部分，激活了烟草病毒的复制蛋白。使用基于结构信息构建的缺失突变体进行的相互作用分析表明，在形成具有RNA5‘-帽活性的蛋白质复合体的过程中，复制蛋白的构象发生了变化。
Eukaryotic positive-strand RNA viruses replicate their genomes in membrane-bound replication complexes composed of viral replication proteins and negative-strand RNA templates. These replication proteins are programmed to exhibit RNA polymerase and other replication-related activities only in replication complexes to avoid inducing double-stranded RNA-mediated host defenses. Host membrane components (e.g. proteins and lipids) should play important roles in the activation of replication proteins. Two host membrane proteins are components of the replication complex and activate the replication proteins of tobamoviruses. Interaction analyses using deletion mutants constructed based on structural information suggest a conformational change in replication proteins during the formation of a protein complex with RNA 5'-capping activity.