Nuclear expression of a cytoplasmic male sterility gene modifies mitochondrial morphology in yeast and plant cells
Nuclear expression of a cytoplasmic male sterility gene modifies mitochondrial morphology in yeast and plant cells
复制标题
DOI：
10.1016/j.plantsci.2005.11.008
复制
发表时间：
2006-04-01
期刊：
影响因子：
5.2
通讯作者：
Budar, F
中科院分区：
文献类型：
--
作者：
Duroc, Y;Gaillard, C;Budar, F
ORF138是萝卜（Raphanus sativus）和芸芥（Brassica sp.）细胞质雄性不育（Ogura cytoplasmic male sterile, cms）的线粒体编码蛋白。为了开发分析cms与不育蛋白结构相关机制的技术工具，我们构建了ORF138、绿色荧光蛋白GFP和ORF138-GFP的基因构建体，以线粒体为目标对编码蛋白进行核表达。这些基因导入酵母，在植物细胞中短暂稳定表达。核编码的ORM 38蛋白靶向线粒体并不会阻止酵母细胞在可发酵或不可发酵培养基上的生长，但会改变酵母和植物细胞线粒体的细胞学外观。然而，核基因和线粒体靶向ORF138的产生并没有引起转基因拟南芥植株的雄性不育。这可能是由于核表达ORF138的亚线粒体位置。2005爱思唯尔爱尔兰有限公司版权所有。
ORF138 is the mitochondrion-encoded protein responsible for Ogura cytoplasmic male sterility (cms) in radish (Raphanus sativus) and Brassica sp. As a means of developing technical tools for analyzing the mechanism of cms in relation to the structure of the sterility protein, we generated gene constructs for ORF138, the green fluorescent protein GFP and an ORF138-GFP fusion, to give nuclear expression with mitochondrial targeting of the encoded proteins. These genes were introduced into the yeast Saccharomyces cerevisiae and were transiently and stably expressed in plant cells. The targeting of nuclear-encoded ORM 38 protein to the mitochondria did not prevent the growth of yeast cells on fermentable or non-fermentable media, but modified the cytological appearance of mitochondria in both yeast and plant cells. However, the production from a nuclear gene and mitochondrial targeting of ORF138 did not induce male sterility in transgenic A. thaliana plants. This may be due to the submitochondrial location of nuclearly expressed ORF138. (c) 2005 Elsevier Ireland Ltd. All rights reserved.