Enhanced release of endothelium-derived factor(s) by chronic increases in blood flow.
Enhanced release of endothelium-derived factor(s) by chronic increases in blood flow.
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通过长期增加血流量来增强内皮衍生因子的释放。
DOI：
10.1152/ajpheart.1988.255.3.h446
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
--
作者：
Miller,VM;Vanhoutte,PM
动静脉瘘引起的慢性血流量增加增强了乙酰胆碱的内皮依赖性松弛。为了确定血管内皮细胞M受体是否发生改变，我们获得了在瘘管手术和假手术的犬股动脉上使用哌仑西平时对乙酰胆碱的浓度-反应曲线。哌仑西平可抑制两条动脉对乙酰胆碱的反应。拮抗剂的PA2(LOG Kb)相同。生物测定系统用于评估内皮细胞衍生的松弛因子的释放。未手术犬的股动脉环(无内皮细胞)在乙酰胆碱刺激过程中与来自造瘘手术动脉内皮细胞的灌流液灌流时更容易松弛。假手术和瘘管手术动脉的无内皮环与未手术的股动脉内皮液灌流后有相同程度的松弛。这些结果表明，在血流量慢性增加的犬动脉中，乙酰胆碱的增强松弛不是由于内皮M受体亚型的改变或潜在的平滑肌对内皮衍生的松弛因子的敏感性的改变(S)。这可能是由于血管内皮细胞衍生的松弛因子(S)在M胆碱激活时释放增加所致。
Chronic increases in blood flow caused by an arteriovenous fistula augment endothelium-dependent relaxations to acetylcholine. To determine whether endothelial muscarinic receptors are altered, concentration-response curves to acetylcholine were obtained in the presence of pirenzepine in fistula- and sham-operated canine femoral arteries. Pirenzepine inhibited the response to acetylcholine in both arteries. The pA2 (log Kb) for the antagonist was the same. A bioassay system was used to assess release of endothelium-derived relaxing factor. Rings of femoral artery (without endothelium) from unoperated dogs relaxed more when superfused with perfusate derived from endothelium of fistula-operated arteries during acetylcholine stimulation. Rings without endothelium of sham- and fistula-operated arteries relaxed to the same extent when superfused with perfusate derived from the endothelium of unoperated femoral arteries. These results suggest that augmented relaxations to acetylcholine in canine arteries where blood flow is chronically elevated do not result from changes in the subtype of endothelial muscarinic receptors or in the sensitivity of the underlying smooth muscle to endothelium-derived relaxing factor(s). They are likely due to increased release of endothelium-derived relaxing factor(s) on muscarinic activation.