Mutations in the Drosophila pushover gene confer increased neuronal excitability and spontaneous synaptic vesicle fusion.
Mutations in the Drosophila pushover gene confer increased neuronal excitability and spontaneous synaptic vesicle fusion.
复制标题
果蝇 Pushover 基因的突变会增加神经元的兴奋性和自发的突触小泡融合。
DOI：
10.1093/genetics/142.4.1215
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Stern,M
中科院分区：
文献类型：
--
作者：
Richards,S;Hillman,T;Stern,M
我们描述了一种被称为“推”的基因的鉴定，它影响神经肌肉交界处的行为和突触传递。携带两种基因突变中的任何一种的成年人表现出迟缓、不协调、有缺陷的逃避反应和雄性不育。发育缺陷的幼虫表现为神经肌肉交界处递质释放增加。特别是，自发性递质释放的频率和神经刺激引起的递质释放量在最低的外部[Ca2+]测试（0.15 mM）时增加了两到三倍。此外，这些突变体对钾通道阻断药物奎尼丁的应用比野生型更敏感：在奎尼丁应用后，推送突变体（而不是野生型）表现出运动轴突的重复放电，导致重复的肌肉突触后电位。因此，推力基因可能影响神经元的兴奋性和递质释放过程。互补测试和重组图谱表明，这些促突变是先前发现的p元素诱导突变的等位基因，这种突变也会导致行为异常和男性不育。
We describe the identification of a gene calledpushover (push), which affects both behavior and synaptic transmission at the neuromuscular junction. Adults carrying either of two mutations inpushexhibit sluggishness, uncoordination, a defective escape response, and male sterility. Larvae defective inpushexhibit increased release of transmitter at the neuromuscular junction. In particular, the frequency of spontaneous transmitter release and the amount of transmitter release evoked by nerve stimulation are each increased two- to threefold inpushmutants at the lowest external [Ca2+] tested (0.15 mM). Furthermore, these mutants are more sensitive than wild type to application of the potassium channel-blocking drug quinidine: following quinidine application,pushmutants, but not wild-type, display repetitive firing of the motor axon, leading to repetitive muscle postsynaptic potentials. Thepushgene thus might affect both neuronal excitability and the transmitter release process. Complementation tests and recombinational mapping suggest that thepushmutations are allelic to a previously identifiedP-element-induced mutation, which also causes behavioral abnormalities and male sterility.