Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies.
Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies.
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DOI：
10.1182/blood.v62.1.124.124
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发表时间：
1983
期刊：
影响因子：
20.3
通讯作者：
R. Andrews;B. Torok-Storb;I. Bernstein
中科院分区：
文献类型：
--
作者：
R. Andrews;B. Torok-Storb;I. Bernstein
在造血系统中，单克隆抗体与抗原决定因子反应，以谱系和阶段限制的方式表达，可用于绘制髓细胞分化图。我们已经生成了一系列单克隆抗体，这些单克隆抗体与髓系干细胞及其后代的髓系相关决定因子发生反应。它们与外周血细胞的反应性通过免疫荧光试验确定，与骨髓细胞的反应性通过荧光激活细胞分选确定，与造血祖细胞的反应性通过细胞毒性试验和荧光激活细胞分选确定。抗体1G10，以前曾报道与粒细胞谱系细胞和一小部分成熟单核细胞反应，被证明与粒细胞-巨噬细胞集落形成单位（CFU-GM）反应。三种以前未被鉴定的抗体（T5A7, L4F3, L1B2）被证明可以与粒细胞和单核细胞反应，并且在荧光激活细胞分选研究中可以检测到不同成熟阶段的粒细胞。这三种抗体也与CFU-GM发生反应，其中两种抗体（L4F3和L1B2）与所有CFU-GM发生反应，而T5A7仅与第7天CFU-GM的一部分发生反应。抗体L4F3也与部分红系爆发形成单位（BFU-E）反应。相比之下，先前报道的抗体5F1，与单核细胞，有核红细胞和血小板反应，被证明与红细胞集落形成单位（CFU-E）反应。讨论了这些抗体在正常和恶性造血研究中的潜在应用。
Within the hematopoietic system, monoclonal antibodies reactive with antigenic determinants, expressed in a lineage- and stage-restricted fashion, can be used to map myeloid differentiation. We have generated a series of monoclonal antibodies that reacts with myeloid-associated determinants on committed myeloid stem cells and their progeny. Their reactivity with peripheral blood cells was identified by immunofluorescence assays, with bone marrow cells by fluorescence-activated cell sorting, and with committed hematopoietic progenitor cells by both cytotoxic assays and fluorescence-activated cell sorting. Antibody 1G10, which has previously been reported to react with cells of the granulocytic lineage and with a minor subset of mature monocytes, was shown to react with granulocyte-macrophage colony-forming units (CFU-GM). Three antibodies not previously characterized (T5A7, L4F3, L1B2) were shown to react with both granulocytic and monocytic cells and in fluorescence-activated cell sorting studies to detectably stain granulocytic cells at different stages of maturation. These three antibodies also react with CFU-GM, two (L4F3 and L1B2) reacting with all CFU-GM, while T5A7 reacts with only a portion of the day 7 CFU-GM. Antibody L4F3 also reacts with a portion of erythroid burst-forming units (BFU-E). In contrast, the previously reported antibody 5F1, which reacts with monocytic cells, nucleated erythroid cells, and platelets, was shown to react with erythroid colony-forming units (CFU-E). Potential applications of these antibodies to studies of normal and malignant hematopoiesis are discussed.