Apelin, an APJ receptor ligand, regulates body adiposity and favors the messenger ribonucleic acid expression of uncoupling proteins in mice
Apelin, an APJ receptor ligand, regulates body adiposity and favors the messenger ribonucleic acid expression of uncoupling proteins in mice
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DOI：
10.1210/en.2006-1270
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发表时间：
2007-06-01
期刊：
影响因子：
4.8
通讯作者：
Yoshimatsu, Hironobu
中科院分区：
文献类型：
--
作者：
Higuchi, Keiko;Masaki, Takayuki;Yoshimatsu, Hironobu
Apelin是APJ受体的内源性配体，已在多种组织中鉴定，包括胃、心脏、骨骼肌和白色脂肪组织。我们试图阐明爱帕琳对C57 BL/6小鼠体内肥胖和解偶联蛋白（UCPs）表达的影响。与对照组相比，以0.1 μ mol/kg center dot d的剂量ip apelin治疗14天，可降低白色脂肪组织的重量以及胰岛素和甘油三酯的血清水平，但不影响摄食量。Apelin治疗还降低了高脂饮食肥胖小鼠的身体肥胖和血清胰岛素和甘油三酯水平。Apelin升高血清脂联素水平，降低血清瘦素水平。此外，爱帕琳肽治疗增加了棕色脂肪组织（BAT）中外周能量消耗标志物UCP 1和骨骼肌中脂肪酸输出调节剂UCP 3的mRNA表达。此外，免疫印迹条带和UCP 1在BAT中的含量的相对密度也高于对照组。此外，apelin治疗增加体温和O-2消耗，降低呼吸商。总之，爱帕琳似乎可以调节瘦小鼠和肥胖小鼠的肥胖和脂质代谢。此外，爱帕琳通过影响小鼠循环脂联素水平、BAT UCP 1表达和能量消耗来调节胰岛素抵抗。
Apelin, the endogenous ligand of the APJ receptor, has been identified in a variety of tissues, including stomach, heart, skeletal muscle, and white adipose tissue. We sought to clarify the effects of apelin on body adiposity and the expression of uncoupling proteins (UCPs) in C57BL/6 mice. Treatment with ip apelin at a dose of 0.1 mu mol/kg center dot d for 14 d decreased the weight of white adipose tissue and serum levels of insulin and triglycerides, compared with controls, without influencing food intake. Apelin treatment also decreased body adiposity and serum levels of insulin and triglycerides in obese mice fed a high-fat diet. Apelin increased the serum adiponectin level and decreased that of leptin. Additionally, apelin treatment increased mRNA expression of UCP1, a marker of peripheral energy expenditure, in brown adipose tissue (BAT) and of UCP3, a regulator of fatty acid export, in skeletal muscle. In addition, immunoblot bands and relative densities of UCP1 content in BAT were also higher in the apelin group than controls. Furthermore, apelin treatment increased body temperature and O-2 consumption and decreased the respiratory quotient. In conclusion, apelin appears to regulate adiposity and lipid metabolism in both lean and obese mice. In addition, apelin regulates insulin resistance by influencing the circulating adiponectin level, the expression of BAT UCP1, and energy expenditure in mice.