Application of a library of near isogenic lines to understand context dependent expression of QTL for grain yield and adaptive traits in bread wheat.
Application of a library of near isogenic lines to understand context dependent expression of QTL for grain yield and adaptive traits in bread wheat.
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DOI：
10.1186/s12870-016-0849-6
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发表时间：
2016-07-19
影响因子：
5.3
通讯作者：
Griffiths S
中科院分区：
文献类型：
--
作者：
Farré A;Sayers L;Leverington-Waite M;Goram R;Orford S;Wingen L;Mumford C;Griffiths S
利用Avalon × Cadenza双单倍体（DH）群体进行的数量性状基因座（QTL）研究共发现了11个决定株高、抽穗期和产量的QTL。本研究的目的是：（i）使用互反多重近等基因系（NIL），在两个亲本背景（Avalon或Cadenza）中比较每对等位基因，来提供对这些QTL的影响的洞察，（ii）通过观察背景效应来量化上位性，以及（iii）预测有利的等位基因组合以开发适应目标环境的上级基因型。为此，在两个生长季节（2012/2013和2013/2014）内测试了553个BC 2近等基因系及其轮回亲本的文库。本研究的结果验证了株高、抽穗期和产量QTL的定位。在株高和抽穗期的6 B QTL、抽穗期和产量的3A QTL和产量的2A QTL上检测到上位性互作。标记辅助回交策略为小麦关键农艺性状QTL的孟德尔效应分析提供了一种有效的方法。研究表明，这些QTL适合于标记辅助选择，精细定位，未来的定位克隆，生理性状解剖。本文的在线版本（doi：10.1186/s12870-016-0849-6）包含补充材料，可供授权用户使用。
Previous quantitative trait loci (QTLs) studies using the Avalon × Cadenza doubled haploid (DH) population identified eleven QTLs determining plant height, heading date and grain yield. The objectives of this study were: (i) to provide insight into the effects of these QTLs using reciprocal multiple near isogenic lines (NILs) with each pair of alleles compared in both parental backgrounds (Avalon or Cadenza), (ii) quantifying epistasis by looking at the background effects and (iii) predict favourable allelic combinations to develop superior genotypes adapted to a target environment. To this aim, a library of 553 BC2 NILs and their recurrent parents were tested over two growing seasons (2012/2013 and 2013/2014). The results obtained in the present study validated the plant height, heading date and grain yield QTLs previously identified. Epistatic interactions were detected for the 6B QTL for plant height and heading date, 3A QTL for heading date and grain yield and 2A QTL for grain yield. The marker assisted backcrossing strategy used provided an efficient method of resolving QTL for key agronomic traits in wheat as Mendelian factors determining possible epistatic interactions. The study shows that these QTLs are amenable to marker assisted selection, fine mapping, future positional cloning, and physiological trait dissection. The online version of this article (doi:10.1186/s12870-016-0849-6) contains supplementary material, which is available to authorized users.