Significant heparin effect on bovine embryo development during sexed in vitro fertilization.
Significant heparin effect on bovine embryo development during sexed in vitro fertilization.
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肝素对性控体外受精期间牛胚胎发育有显着影响。
DOI：
10.1262/jrd.2016-142
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发表时间：
2017-04-21
期刊：
影响因子：
--
通讯作者：
DU F
中科院分区：
文献类型：
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作者：
An LY;Chaubal SA;Liu Y;Chen Y;Nedambale TL;Xu J;Xue F;Moreno JF;Tao S;Presicce GA;DU F
本研究旨在探讨体外受精（IVF）过程中不同肝素浓度对牛胚胎发育和胚胎移植（ET）后发育的影响。共有9156个卵母细胞用于IVF，来自4头公牛的分选和未分选精子具有不同的肝素需求，以实现体外发育的最高速率。然而，当使用最佳肝素浓度时，（40至80 µg/ml），X-分选精子的性能（0.3 × 106/ml/IVF液滴），根据囊胚发育判断（公牛A、B、C和D：分别为25.2、19.7、25.1和9.8%），显著增加，并且在四头公牛中，在某些肝素浓度下，囊胚率与未分选精子的囊胚率相当。我们确定，当使用40 µg/ml的肝素浓度时，来自所有四头公牛的分选精子可以实现接近最佳的囊胚发育。当肝素浓度分别为10 μg/ml（n = 236）、20 μg/ml（n = 189）和40 μg/ml（n = 305）时，ET后70 d的妊娠率为39.1 ~ 40.3%（P> 0.05），产犊率为34.4 ~ 35.1%（P> 0.05）。我们的研究表明，尽管不同公牛的分选精子在肝素浓度范围内表现最佳，但一般接受的肝素浓度为40 μg/ml可用于性别IVF。当通过卵子拾取和IVF进行性别鉴定胚胎生产是遗传育种计划的重要组成部分时，这种改进是有益的。
The aim of this study was to investigate the effect of different heparin concentrations in the course of sexed in vitro fertilization (IVF), on bovine embryonic development and development to term following embryo transfer (ET). With a total of 9156 oocytes for IVF, sorted as well as unsorted sperm from four bulls had different heparin requirements for achieving the highest rate of development in vitro. However, when optimal heparin concentrations were used (40 to 80 µg/ml), the performance of X-sorted sperm (0.3 × 106/ml/IVF droplet) from all four bulls, as judged by blastocyst development (Bulls A, B, C, and D: 25.2, 19.7, 25.1, and 9.8%, respectively), was significantly increased, and the blastocyst rate was comparable to that observed with unsorted sperm at certain heparin concentrations within the four bulls. We determined that near-optimal blastocyst development was possible with sorted sperm from all four bulls, when a heparin concentration of 40 µg/ml was used. Pregnancy rates at d 70 post ET ranged from 39.1 to 40.3% (P > 0.05), and the calving rates ranged from 34.4 to 35.1% (P > 0.05), when heparin was used at a concentration of 10 μg/ml (n = 236), 20 μg/ml (n = 189), and 40 μg/ml (n = 305), respectively. Our study demonstrates that, although the sorted sperm of different bulls performed optimally over a range of heparin concentrations, a generally accepted heparin concentration of 40 µg/ml can be set for sexed IVF. This improvement is beneficial when sexed embryo production by ovum pickup and IVF is an essential component of genetic breeding programs.