Center surround receptive field structure of cone bipolar cells in primate retina
Center surround receptive field structure of cone bipolar cells in primate retina
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DOI：
10.1016/s0042-6989(00)00039-0
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发表时间：
2000-01-01
期刊：
影响因子：
1.8
通讯作者：
Lee, B
中科院分区：
文献类型：
--
作者：
Dacey, D;Packer, OS;Lee, B
在非哺乳类脊椎动物中，视网膜双极细胞显示中心-周围感受野组织。在哺乳动物中，来自双极细胞的记录是罕见的，并且没有显示出清晰的周围环境。在这里，我们报告的中心周围的感受野确定锥双极细胞在猕猴视网膜。在外周视网膜，锥双极细胞核标记在体外与diamidino-phenylindole（DAPI），有针对性的记录下显微镜控制，和解剖学鉴定细胞内染色。所鉴定的细胞包括接触多个视锥的“弥漫性”双极细胞和接触单个视锥的“侏儒”双极细胞。闪烁的斑点和annuli的反应揭示了一个明确的包围：超极化（OFF）和去极化（ON）细胞反应与反极性环状刺激。中心和周围的尺寸计算12双极细胞的空间频率响应漂移，正弦亮度调制光栅。频率响应是带通的，并能很好地用差分高斯感受保持模型拟合。中心直径都比已知的树突状树直径大两到三倍，在视网膜周边的弥漫性和侏儒双极细胞。在一个实例中，细胞内染色显示示踪剂之间的记录细胞和它最近的邻居，这表明同型电耦合可能有助于感受野中心的大小。周围的直径比中心大大约十倍，在大多数情况下，中心与周围强度的比率接近1。我们认为，主要的灵长类动物神经节细胞类型的中心周围的感受野建立在双极细胞，可能是由外部视网膜的电路。(C)2000爱思唯尔科技有限公司版权所有。
In non-mammalian vertebrates, retinal bipolar cells show center-surround receptive field organization. In mammals, recordings from bipolar cells are rare and have not revealed a clear surround. Here we report center-surround receptive fields of identified cone bipolar cells in the macaque monkey retina. In the peripheral retina, cone bipolar cell nuclei were labeled in vitro with diamidino-phenylindole (DAPI), targeted for recording under microscopic control, and anatomically identified by intracellular staining. Identified cells included 'diffuse' bipolar cells, which contact multiple cones, and 'midget' bipolar cells, which contact a single cone. Responses to flickering spots and annuli revealed a clear surround: both hyperpolarizing (OFF) and depolarizing (ON) cells responded with reversed polarity to annular stimuli. Center and surround dimensions were calculated for 12 bipolar cells from the spatial frequency response to drifting, sinusoidal luminance modulated gratings. The frequency response was bandpass and well fit by a difference of Gaussians receptive held model. Center diameters were all two to three times larger than known dendritic tree diameters for both diffuse and midget bipolar cells in the retinal periphery. In one instance intracellular staining revealed tracer spread between a recorded cell and its nearest neighbors, suggesting that homotypic electrical coupling may contribute to receptive field center size. Surrounds were around ten times larger in diameter than centers and in most cases the ratio of center to surround strength was similar to 1. We suggest that the center-surround receptive fields of the major primate ganglion cell types are established at the bipolar cell, probably by the circuitry of the outer retina. (C) 2000 Elsevier Science Ltd. All rights reserved.