Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation
Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation
复制标题
DOI：
10.1242/dev.186957
复制
发表时间：
2020-04-01
期刊：
影响因子：
4.6
通讯作者：
Mirouse, Vincent
中科院分区：
文献类型：
--
作者：
Campos, Fabiana Cerqueira;Dennis, Cynthia;Mirouse, Vincent
细胞外基质如何参与组织形态发生仍是一个悬而未决的问题。在果蝇的卵泡中，已经提出在卵泡细胞基底结构域的Fat 2依赖性平面极化之后，定向基底膜（BM）原纤维和F-肌动蛋白应力纤维限制卵泡生长，促进其轴向伸长。然而，BM原纤维和应力纤维之间的关系以及它们各自对伸长率的影响尚不清楚。我们发现Dystroglycan（Dg）和Dystrophin（Dys）参与BM纤维沉积。此外，它们还通过局部作用并平行于Fat 2来定向应力纤维。重要的是，Dg-Dys复合物介导的细胞自主控制的F-肌动蛋白纤维的方向依赖于前BM原纤维沉积，表明两个不同的，但相互依存的功能。因此，Dg-Dys复合物作为上皮基底结构域的重要组织者起作用，调节F-肌动蛋白和BM。此外，BM原纤维作为一个持久的线索，应力纤维的方向是伸长的主要效应。
How extracellular matrix contributes to tissue morphogenesis is still an open question. In the Drosophila ovarian follicle, it has been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils and stress fibers and their respective impact on elongation are unclear. We found that Dystroglycan (Dg) and Dystrophin (Dys) are involved in BM fibril deposition. Moreover, they also orient stress fibers, by acting locally and in parallel to Fat2. Importantly, Dg-Dys complex-mediated cell-autonomous control of F-actin fiber orientation relies on the preceding BM fibril deposition, indicating two distinct but interdependent functions. Thus, the Dg-Dys complex works as a crucial organizer of the epithelial basal domain, regulating both F-actin and BM. Furthermore, BM fibrils act as a persistent cue for the orientation of stress fibers that are the main effector of elongation.