Identification of a novel brain-specific and reelin-regulated gene that encodes a protein colocalized with synapsin
Identification of a novel brain-specific and reelin-regulated gene that encodes a protein colocalized with synapsin
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DOI：
10.1111/j.1460-9568.2004.03473.x
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发表时间：
2004-08-01
影响因子：
3.4
通讯作者：
Simonneau, M
中科院分区：
文献类型：
--
作者：
Kuvbachieva, A;Bestel, AM;Simonneau, M
我们进行了一项在正常小鼠和Reeler突变体中差异表达的基因的筛选，这些基因的特征是由于Reelin信号缺陷而导致的神经元异常迁移和轴突部署。一个新的基因，暂时命名为c61，在Reelin缺陷的小鼠胚胎脑RNA中过表达。C61编码一个3.7kb的mRNA，它是大脑特异的，受发育调节，在分化的神经元中主要表达。预测的蛋白质全长664个氨基酸，含有LAG1和Ezrin/Radiin/Moesin-myosin-细丝基序，提示它可能具有细胞内接头的功能。从胎龄14.5天到出生，C61在所有神经元分化区域都有高表达，其中以端脑皮质板层和嗅球内的二尖瓣细胞表达最强。当以GFP融合蛋白的形式在转基因的非神经元细胞和原代神经元中表达时，该蛋白分别定位于核膜或轴突突起，表明在轴突交通或信号传递中具有功能。
We carried out a screening of genes that are differentially expressed in normal mice and reeler mutants and are characterized by abnormal neuronal migration and neurite deployment due to defective Reelin signalling. A novel gene, provisionally named C61, was overexpressed in Reelin-deficient embryonic mouse brain RNA. C61 encodes a 3.7 kb mRNA that is brain specific and developmentally regulated, with predominant expression in differentiating neurons. The predicted protein is 664 amino acids long, and contains LAG1 and Ezrin/Radixin/Moesin-Myosin-Filament motifs, suggesting that it may function as an intracellular adaptor. From E14.5 to birth, C61 was highly expressed in all neuronal differentiation fields, with the highest signal in the telencephalic cortical plate and mitral cells in the olfactory bulb. When expressed as a GFP fusion protein in transfected non-neuronal cells and primary neurons, this protein localizes, respectively, to the nuclear membrane or axonal outgrowths, indicating a function in axonal traffic or signalling.