Decolorization of Direct Blue GLL with enhanced lignin peroxidase enzyme production in Comamonas sp UVS
Decolorization of Direct Blue GLL with enhanced lignin peroxidase enzyme production in Comamonas sp UVS
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DOI：
10.1002/jctb.2017
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发表时间：
2009-01-01
影响因子：
3.4
通讯作者：
Govindwar, Sanjay P.
中科院分区：
文献类型：
--
作者：
Jadhav, Umesh U.;Dawkar, Vishal V.;Govindwar, Sanjay P.
背景技术背景：研究了利用不同培养基和木质纤维素废料培养的丛毛单胞菌UVS产木质素过氧化物酶（LiP）及其对直接蓝GILL（DBGLL）的脱色效果。甘蔗渣粉沿着酵母提取物诱导Lip活性。在静态条件下，丛毛单胞菌（Comamonas sp UVS）对DBGLL染料（50 mg L ~（-1））的脱色作用在13 h内完成.在55 h内，该菌可降解300 mg L-1的染料。最大脱色速率（Vmax）为12.41 +/- 0.55 mg染料g细胞（-1）h（-1），米氏常数（Km）为6.20 +/- 0.27 mg L-1。结论：利用农副产品提高木质素降解酶的活性是一种经济有效的方法。它还解决了农业残留物的处置问题。该体系可应用于不同难降解化合物的降解。(C)2008年化学工业协会
BACKGROUND: The present work aims to study the production of lignin peroxidase (LiP) enzyme by Comamonas sp UVS using various media, and lignocellulosic waste materials, and its effect on decolorization of Direct Blue GILL (DBGLL).RESULTS: Yeast extract medium was found to be more effective for the production of Lip and also for the decolorization of DBGLL. The bagasse powder along with yeast extract induced Lip activity. Comamonas sp UVS decolorized DBGLL dye (50 mg L-1) within 13 h at static condition in YE broth. It could degrade up to 300 mg L-1 of dye within 55 h. The maximum rate (Vmax) of decolorization was 12.41 +/- 0.55 mg dye g cell(-1) h(-1) with the Michaelis constant (Km) value as 6.20 +/- 0.27 mg L-1. The biodegradation was monitored by UV-Vis, GC-MS and HPLC.CONCLUSION: The use of agricultural by-products for the activity enhancement of the ligninolytic enzymes is a cost effective process. It also resolves the problem of the disposal of agro-residues. This system can be applied for the degradation of different recalcitrant compounds. (C) 2008 Society of Chemical Industry