Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system
Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system
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DOI：
10.1128/aem.01291-08
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发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Henderson, D. P.
中科院分区：
文献类型：
--
作者：
Villarreal, D. M.;Phillips, C. L.;Henderson, D. P.
要在大肠杆菌中产生重组血红蛋白，必须有足够的细胞内血红素存在，否则蛋白质就会不正确地折叠并被降解。在本研究中，人血红蛋白基因和志贺单胞菌血红素运输基因的共表达促进了在含血红素培养基中生长的大肠杆菌BL21（DE3）重组血红蛋白的产生。
To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.