A centrifugation cell adhesion assay for high-throughput screening of biomaterial surfaces
A centrifugation cell adhesion assay for high-throughput screening of biomaterial surfaces
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DOI：
10.1002/jbm.a.10122
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发表时间：
2003-10-01
影响因子：
4.9
通讯作者：
García, AJ
中科院分区：
文献类型：
--
作者：
Reyes, CD;García, AJ
细胞粘附的定量分析对于理解生理现象和设计生物材料、植入物表面和组织工程支架是必不可少的。用于评估生物材料表面的最常见的细胞粘附测定缺乏灵敏度和再现性和/或需要专门的设备和技能密集型操作。我们描述了一种改良的离心细胞粘附试验，使用简单方便的技术与标准的实验室设备，并提供可靠的，定量测量细胞粘附。该离心试验对大量粘附细胞施加受控且均匀的分离力，为定量细胞粘附提供了可靠的统计数据。该系统的生物材料表面的设计和表征的适用性，通过使用不同的包被蛋白，细胞类型，接种时间和相对离心力（RCF）的基板上的细胞粘附进行评估。结果证实，这种离心细胞粘附试验代表了一种简单，方便，标准的方法，用于高通量表征各种生物材料表面和条件。(C)2003 Wiley Periodicals，Inc.
A quantitative analysis of cell adhesion is essential in understanding physiological phenomena and designing biomaterials, implant surfaces, and tissue-engineering scaffolds. The most common cell adhesion assays used to evaluate biomaterial surfaces lack sensitivity and reproducibility and/or require specialized equipment and skill-intensive operation. We describe a modified centrifugation cell adhesion assay that uses simple and convenient techniques with standard laboratory equipment and provides reliable, quantitative measurements of cell adhesion. This centrifugation assay applies controlled and uniform detachment forces to a large population of adherent cells, providing robust statistics for quantifying cell adhesion. The applicability of this system to the design and characterization of biomaterial surfaces is shown by evaluating cell adhesion on substrates using different coating proteins, cell types, seeding times, and relative centrifugal forces (RCF). Results verify that this centrifugation cell adhesion assay represents a simple, convenient, and standard method for high-throughput characterization of a variety of biomaterial surfaces and conditions. (C) 2003 Wiley Periodicals, Inc.