Antimicrobial actions of benzimidazoles against oral streptococci.
Antimicrobial actions of benzimidazoles against oral streptococci.
复制标题
苯并咪唑对口腔链球菌的抗菌作用。
DOI：
10.1111/j.1399-302x.2004.00197.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Marquis,RE
中科院分区：
文献类型：
--
作者：
Nguyen,PTM;Baldeck,JD;Olsson,J;Marquis,RE
Background/aim:Benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton‐pump inhibitors to control stomach acid secretion and also have antimicrobial actions againstHelicobacter pylori. Our objective was to determine whether they are potentially useful antimicrobials against oral bacteria.Methods:Streptococcus mutanswas our main test organism. It was grown in suspension cultures and biofilms. Standard physiologic assays were used to assess inhibitory actions of benzimidazoles.Results:Benzimidazoles inhibited acid production byS. mutansin suspensions or biofilms. In pH‐drop experiments, lansoprazole at a level of only 0.025 mmirreversibly inhibited acid production from glycolysis. Cell uptake of lansoprazole was found to be very pH sensitive and occurred mainly at pH values below about 5, indicating that the protonated form was taken up. Lansoprazole inhibition of glycolysis could be blocked by 2‐mercaptoethanol, which suggests that disulfide bonds form between benzimidazoles and protein targets. Identified targets for benzimidazole inhibition included the phosphoenolpyruvate : sugar phosphotransferase system, the glycolytic enzymes aldolase, glyceraldehyde‐3‐phosphate dehydrogenase, and lactic dehydrogenase, and enzymes such as urease and arginine deiminase. Lansoprazole increased proton permeabilities ofS. mutanscells but did not inhibit F‐ATPases. Although cells in biofilms were somewhat less sensitive to the agents than those in suspensions, biofilm glycolysis was still markedly inhibited by 0.1 mmlansoprazole. Benzimidazoles are bactericidal, and the oral anaerobesFusobacterium nucleatumandPrevotella intermediawere more sensitive to killing than wasS. mutans.Conclusion:Benzimidazoles appear to be useful inhibitors of oral bacteria in acid environments such as progressing caries lesions.
