Liquid-based cytology in the fine needle aspiration of parathyroid lesions: a comparison study with the conventional smear, ThinPrep, and SurePath.
Liquid-based cytology in the fine needle aspiration of parathyroid lesions: a comparison study with the conventional smear, ThinPrep, and SurePath.
复制标题
甲状旁腺病变细针抽吸液基细胞学检查：与传统涂片、ThinPrep 和 SurePath 的比较研究。
DOI：
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发表时间：
2015
影响因子：
1.4
通讯作者：
C. Jung
中科院分区：
文献类型：
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作者：
G. Park;Sung Hak Lee;S. Jung;C. Jung
液基细胞学(LBC)已逐渐用于评估细针吸取(FNA)标本。然而，有限的研究检查了甲状旁腺病变的FNA中的LBC。我们回顾了24例甲状旁腺病变的FNA标本，其中常规涂片6例，ThinPrep法12例，SurePath法6例。取18例LBC作细胞块制备和甲状旁腺激素(PTH)免疫组织化学染色。与常规涂片标本相比，LBC标本更常见的表现为细胞密度变化、微滤泡结构、泡状或空泡状细胞质、边界清楚的小而圆的细胞。与其他方法制备的样品相比，ThinPrep样品显示出干净的背景，分离的细胞和裸露的核较少。与ThinPrep样本相比，SurePath样本显示背景中有许多白细胞，更多散乱的单个细胞和裸露的核。用这3种方法制备的标本通常呈胶状物质，但不包含致密的球状胶体结构。LBC样本背景中的白细胞是估计细胞大小的有用指标。甲状旁腺细胞的核大小与背景中的炎性细胞相似或更小。细胞块切片显示甲状旁腺组织有明确的组织学特征，甲状旁腺激素免疫组织化学染色呈强阳性。了解使用ThinPrep和SurePath方法制备的甲状旁腺FNA标本的这些细胞学特征可能有助于防止误诊。甲状旁腺病变的确诊应行细胞块制备和甲状旁腺激素免疫组织化学染色。
Liquid-based cytology (LBC) has been progressively used for evaluating fine needle aspiration (FNA) specimens. However, limited studies have examined LBC in FNA of parathyroid lesions. We retrospectively reviewed 24 FNA specimens of parathyroid lesions, including 6 specimens prepared by conventional smear, 12 specimens prepared using ThinPrep method, and 6 specimens prepared using SurePath method. The 18 LBC specimens were also used for cell block preparation and immunostaining for parathyroid hormone (PTH). LBC specimens more frequently showed variable cellularity; microfollicular structure; bubbly or vacuolated cytoplasm; and small, round cells with distinct borders compared to specimens prepared by conventional smear. ThinPrep specimens showed a clean background and fewer isolated cells and naked nuclei compared to specimens prepared using the other methods. SurePath specimens showed many white blood cells in the background and more scattered single cells and naked nuclei compared to ThinPrep specimens. Specimens prepared using the 3 methods often showed colloid-like material but did not contain dense globular colloidal structures. White blood cells in the background of LBC specimens serve as useful indicators for estimating cell size. The nuclear size of parathyroid cells was similar to or smaller than that of inflammatory cells in the background. Cell block sections showed definite histological features of the parathyroid tissue and strong positive immunostaining for PTH. Awareness of these cytologic features of parathyroid FNA specimens prepared using ThinPrep and SurePath methods may help in preventing misdiagnosis. Cell block preparation and PTH immunostaining should be performed for the definitive diagnosis of parathyroid lesions.