IDENTIFICATION OF A NEW DISULFIDE BONDED COLLAGEN FROM CARTILAGE
IDENTIFICATION OF A NEW DISULFIDE BONDED COLLAGEN FROM CARTILAGE
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DOI：
10.1016/0014-5793(80)81265-8
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发表时间：
1980-01-01
期刊：
影响因子：
3.5
通讯作者：
BAILEY, AJ
中科院分区：
文献类型：
--
作者：
SHIMOKOMAKI, M;DUANCE, VC;BAILEY, AJ
II型是在透明软骨中发现的主要胶原蛋白[1，2]，但已分离出一小部分3种额外的多肽链并进行了部分表征。据报道，两个新组分lo和2 o的组成[3]类似于V型CUA和aB链[4]，而第三个链3a显示类似于从牛软骨制备的α ~（11）minor型[5]。这3条链的分子组成下降，软骨的显微分析未能揭示这些新胶原类型的空间分布。尽管在各种组织中已经报道了几种其他类型的纤维胶原[6]，并且最近在基底膜胶原中报道了一些二硫键结合的分子[7-91]，但是这些都没有在软骨中被鉴定。该通讯报道了从新生猪透明软骨中分离和部分表征一种新型胶原蛋白类型，与la、2ar和3 o组分不同[3]，该胶原蛋白被mer~ ptoet~ nol还原为较小的组分，表明该链通过链内二硫键稳定。
Type II is the predominant collagen found in hyaline cartilage [1, 2] but a small proportion of 3 additional polypeptide chains have been isolated and partially characterised. The composition of two of the new components, lo and 2o was reported [3] to resemble the CUA and aB chains of type V [4], whilst the third chain, 3a, was shown to be similar to type (~(11) minor prepared from bovine cartilage [5]. The molecular composition of these 3 chains is down, and micro-analysis of the cartilage failed to reveal a spatial distribution of these new collagen types. Although several other types of fibrous collagen have been reported in various tissues [6], and more recently some disulphide bonded molecules in basement membrane collagen [7-91, none of these has been identified in cartilage. This communication reports the isolation and partial characterisation of a novel collagen type from neonatal pig hyaline cartilage which, unlike the la, 2ar and 3o components [3], is reduced to smaller fra~ ents by mer~ ptoet~ nol, indicating that the chain is stabilised by intra-chain disulphide bonds.